14-613-M

Brk Protein, active, 10 µg

• UNSPSCコード: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

特性

• リコンビナント: expressed in baculovirus infected Sf21 cells
• 品質水準: 300
• 詳細: protein target BRK
• 精製方法: (Ni²⁺/NTA-agarose)
• テクニック: activity assay: suitable
• UniProtアクセッション番号: Q13882
• 遺伝子情報: human ... PTK6(5753)

詳細

詳細

FUNCTION: SwissProt: Q13882 # Phosphorylates KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. May function as an intracellular signal transducer in epithelial tissues. Overexpression in mammary cells leads to mitogenically sensitization to EGF, and results in a partially transformed phenotype. Its presence in the nucleus appears to be linked to suppression of tumor progression.
SIZE: 451 amino acids; 51834 Da
SUBUNIT: Interacts with GAP-A.p65 (By similarity). Interacts with KHDRBS1.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. In secretory epithelial cells from prostate adenocarcinoma, nuclear localization is higher in low-grade and lower in high-grade regions of the tumors.
TISSUE SPECIFICITY: Epithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Expressed at low level in some breast tumors, but not in normal breast. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
PTM: Autophosphorylated. The phosphorylation of Tyr-447 may lead to the autoinhibition of the enzyme.
SIMILARITY: SwissProt: Q13882 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. BRK/PTK6/SIK subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.

包装

Also available in 10 μg size (cat#14-613) and in bulk (cat#14-613-K).

品質

Routinely evaluated by phosphorylation of Poly (Glu4-Tyr)

routinely evaluated by phosphorylation of Poly (Glu4-Tyr)

物性

55.3kDa

保管および安定性

6 months at -70°C

アナリシスノート

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.