14-429-M

MEK1 Protein, active, 10 µg

Active, N-terminal GST & C-terminal His6-tagged, recombinant full-length human MEK1, for use in Kinase Assays.

• 別名: CFC3, MAP2K1, MAPKK1, MEL, MKK1, PRKMK1, mitogen-activated protein kinase kinase 1
• UNSPSCコード: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

特性

• 由来生物: human
• 品質水準: 100
• リコンビナント: expressed in E. coli
• 製品種目: Upstate^®
• フォーム: liquid
• 比活性: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
• シェルフライフ: 1 yr
• 分子量: M_w 71 kDa
• 精製方法: (Purified using glutathione agarose followed by Ni2+/NTA agarose, activated using c-Raf and the re-purified using Ni2+/NTA agarose)
• 化学種の反応性: human
• メーカー／製品名: Upstate^®
• テクニック: activity assay: suitable (kinase)
• 溶解性: soluble
• アクセッション番号: (FUNCTION: SwissProt: Q02750 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases. ); NM_002755.2
• NCBIアクセッション番号: NM_002755.2
• UniProtアクセッション番号: Q02750
• 保管温度: −70°C
• 遺伝子情報: human ... MAP2K1(5604)

詳細

詳細

Research area: Cell Signaling Protein Target: MEK1 Target Sub-Family: STE

N-terminal GST and Cterminal His6-tagged, recombinant full-length human MEK1

生物化学的／生理学的作用

Mitogen-activated protein kinase kinase 1 (MEK1) is a hydrophilic non-receptor protein kinase that shows ubiquitous expression across different cell types. It serves as a critical component of the RAS-RAF-MEK-ERK signal transduction cascade, also known as the extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) cascade. This signaling pathway plays a crucial role in regulating various biological processes, including apoptosis, cell cycle progression, cell migration, differentiation, metabolism, and proliferation. Additionally, MEK1 protein contributes to the development of the extra-embryonic ectoderm during placentogenesis. Somatic mutations in the MEK1 gene have been observed in individuals with melanoma, a form of skin cancer.

包装

Also available in 10 μg size and in bulk

Also available in 250μg size as catalog # 14-429M - Call for pricing and availability.

品質

routinely evaluated by phosphorylation of MBP

ターゲットの説明

MEK1

物理的形状

Purified using glutathione agarose followed by Ni2+/NTA agarose, activated using c-Raf and the re-purified using Ni2+/NTA agarose

保管および安定性

1 year at -70°C

その他情報

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H317
• 注意書き: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危険有害性の分類: Skin Sens. 1
• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(℃): Not applicable