由来生物
human
品質水準
リコンビナント
expressed in Sf21 cells
分子量
α-subunit Mw 49 kDa (heterotetramer; α2β2)
β subunit Mw 53 kDa (heterotetramer; α2β2)
メーカー/製品名
Upstate®
テクニック
activity assay: suitable (kinase)
NCBIアクセッション番号
UniProtアクセッション番号
詳細
Product Source: Expressed in Sf21 insect cells.
Recombinant full-length human Casein Kinase 2 protein, containing an &alpyha;-subunit N-terminal 6His tag and a β-subunit N-terminal GST tag.
生物化学的/生理学的作用
Protein Target: CK2
包装
2 vials at 5μg each
Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-197M when ordering the 250μg size.
Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-197M when ordering the 250μg size.
品質
Routinely evaluated by phosphorylation of the Casein Kinase 2 Substrate Peptide.
物理的形状
Sequential chromatography using heparin Sepharose™ and Mono Q anion exchange resin
20mM MOPS, pH 7.4, 1mM EDTA, 0.1% (v/v) Triton X-100, 0.3% 2-mercaptoethanol, 1mM benzamidine, 50% glycerol.
保管および安定性
1 year at -20°C.
その他情報
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
法的情報
Sepharose is a trademark of Cytiva
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
In vitro phosphorylation of the polyomavirus major capsid protein VP1 on serine 66 by casein kinase II
The Journal of Biological Chemistry, 270, 26006-26011 (1995)
Casein kinase II beta-subunit inhibits the activity of the catalytic alpha-subunit in the absence of salt.
Biochimica et Biophysica Acta, 1203, 282-289 (1993)
European journal of biochemistry, 229(3), 703-709 (1995-05-01)
Casein kinase II, cyclin-dependent kinases, and glycogen synthase kinase-3 are members of the protein kinase subfamily with a prominent insert in domain X of their catalytic subunit sequence. The function of the insert sequence in casein kinase II was investigated
The Biochemical journal, 352 Pt 1, 145-154 (2000-11-04)
Stress-activated protein kinase 1 (SAPK1), also called c-Jun N-terminal kinase (JNK), becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif
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