13-110

MBP, Dephosphorylated

• 別名: MBP protein
• UNSPSCコード: 12352200
• eCl@ss: 32160405
• NACRES: NA.41

特性

• 由来生物: bovine
• 品質水準: 100
• 形状: liquid
• シェルフライフ: 2 yr
• メーカー／製品名: Upstate^®
• テクニック: activity assay: suitable
• 溶解性: water: soluble
• NCBIアクセッション番号: NM_001025081.1; NM_001025090.1; NM_001025092.1; NM_001025094.1; NM_001025098.1; NM_001025100.1; NM_001025101.1; NM_002385.2
• UniProtアクセッション番号: P02687
• 輸送温度: wet ice
• 保管温度: −20°C
• 遺伝子情報: bovine ... MBP(618684)

詳細

詳細

Research Area: Cell Signaling
Myelin basic protein (MBP) constitutes approximately 30% of the total protein and accounts for about 10% of the dry weight of myelin. It is primarily associated with the cytosolic side of the oligodendrocyte (OL) membrane, largely through electrostatic interactions with acidic lipids. This protein is distributed throughout compact internodal myelin. MBP consists of multiple post-translationally modified isomers with varying charges, some of which arise from phosphorylation at several sites by different kinases, including mitogen-activated protein kinase (MAPK).

アプリケーション

Myelin basic protein purified from bovine brain and de-phosphorylated using Lambda protein phosphatase. No detectable endogenous phosphorylation as determined by immunoblotting 1 microgram of MBP with 1 microgram/ml anti phospho MBP.

生物化学的／生理学的作用

Myelin basic protein (MBP) is distributed throughout compact internodal myelin. It binds to negatively charged lipids on the cytosolic surface of oligodendrocyte membranes, likely facilitating the adhesion of these surfaces in the multilayered myelin sheath. MBP is characterized as an intrinsically unstructured protein, providing it with the flexibility to bind to a charged surface along its entire length and adapt its local conformation as needed to optimize binding to various targets. Such proteins often function as multifunctional regulatory proteins.
MBP interacts with polyanions such as actin filaments and microtubules in vitro and binds to other ligands such as Ca²⁺-calmodulin (CaM). MBP binds to G-actin, inducing its polymerization, and it bundles actin filaments. It also polymerizes actin, bundles F-actin filaments, and binds actin filaments to lipid bilayers through electrostatic interactions. Phosphorylation and dephosphorylation of MBP occur in the myelin sheath in response to electrical activity in the brain.

品質

routinely evaluated as a substrate in a kinase assay using MAP Kinase 2/Erk2 (Catalog # 14-173)

物理的形状

10mM MOPS, pH 7.0, 128mM MnCl2, 641mM EDTA, 1.134μg inactive lambda phosphatase and 0.05% sodium azide

保管および安定性

2 years at -20°C

その他情報

This product is derived from bovine source. Export of this product to certain countries may be restricted. Please contact Customer Service or your local distributor to inquire about product availability and export options.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable