ligand
(Trimethylammoniumethyl)
品質水準
物質
polypropylene housing
製品種目
Fractogel®
形状
prepacked column
パラメーター
≤8 bar pressure
~240 cm/hr max. flow rate
ベッド長
100 mm
ベッド容積
5 mL
カラム内径
8 mm
容量
5 mL
マトリックス活性基
Methacrylate
平均粒径
40-90 μm
キャパシティ
~100 mg, BSA binding capacity
アプリケーション
gene therapy
mAb
recombinant protein
分離法
anion exchange
保管温度
15-25°C
詳細
8 x 100 mm
MiniChrom Pre-packed Columns with Fractogel® cation exchange resins are designed for the rapid evaluation of resins for bioprocess development. The results obtained for biomolecule separations are generally comparable to the results achieved using laboratory-scale columns (e.g. 100 x 16 mm ID).
In bioprocess design, the MiniChrom columns can be used to rapidly determine whether a target biomolecule is bound to and can be eluted from the Fractogel® resin, as well as determine whether an appropriate target yield may be achieved and whether major contaminants could potentially be removed.
MiniChrom columns are designed for process development, method optimization and parameter screening as well as sample preparation. MiniChrom columns are quality checked for HETP and asymmetry, providing superior reproducibility, excellent scalability, convenient comparison of different chromatography media.
MiniChrom columns are made from biocompatible polypropylene (PP) and polyethylene (PE) and are available in 1 and 5 mL bed volumes. The 8mm diameter columns allow for scale-up from 1 to 5mL column volumes, with a constant internal diameter. These columns are compatible with any chromatographic work station, HPLC system or peristaltic pump.
In bioprocess design, the MiniChrom columns can be used to rapidly determine whether a target biomolecule is bound to and can be eluted from the Fractogel® resin, as well as determine whether an appropriate target yield may be achieved and whether major contaminants could potentially be removed.
MiniChrom columns are designed for process development, method optimization and parameter screening as well as sample preparation. MiniChrom columns are quality checked for HETP and asymmetry, providing superior reproducibility, excellent scalability, convenient comparison of different chromatography media.
MiniChrom columns are made from biocompatible polypropylene (PP) and polyethylene (PE) and are available in 1 and 5 mL bed volumes. The 8mm diameter columns allow for scale-up from 1 to 5mL column volumes, with a constant internal diameter. These columns are compatible with any chromatographic work station, HPLC system or peristaltic pump.
特徴および利点
MiniChrom Column Fractogel® TMAE (M), 5 ml enables:
- High protein loading conditions resulting in good selectivity and resolution with the tentacle technology
- Excellent binding to large viruses at increased loading
- Homogenous binding with high selectivity and purity
- Reduce operating costs and resin replacement with enduring durability - allowing for multiple cycles of column regeneration and sanitization
- Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution
法的情報
FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany
試験成績書（COA）
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