07-2129

Anti-phospho-Abi-1 (Ser225) Antibody

from rabbit, purified by affinity chromatography

• 別名: Abl interactor 1, Abelson interactor 1, Abi-1, Abl-binding protein 4, AblBP4, Eps8 SH3 domain-binding protein, Eps8-binding protein, Nap1-binding protein, Nap1BP, Spectrin SH3 domain-binding protein 1, e3B1
• UNSPSCコード: 12352203
• eCl@ss: 32160702

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: monkey, human
• 化学種の反応性（ホモロジーによる予測）: rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
• テクニック: western blot: suitable
• NCBIアクセッション番号: NP_001012768
• UniProtアクセッション番号: Q8IZP0
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: phosphorylation (pSer225)
• 遺伝子情報: human ... ABI1(10006)

詳細

詳細

Abl interactor 1 (Abelson interactor 1) is a member of the family of Abl-interactors and is highly expressed in the brain. Abl interactor 1 is involved in the signaling of ABL1 and ABL2 nonreceptor tyrosine kinases, and the EGF-receptor. The Abl interactor 1 protein contains SH3 domains, and may function as an adaptor protein; It forms a trimeric complex with EPS8 and SOS1 in the transduction of signals from Ras to Rac. Previous studies have suggested that Abl interactor 1 via the Ras and Rac pathways may regulate cell proliferation, cell adhesion, and actin remodeling. Abl interactor 1 is also investigated for its role in tumor suppression.

特異性

This antibody recognizes Abi-1 phosphorylated at Ser225.

免疫原

Epitope: Phosphorylated Ser225

KLH-conjugated linear peptide corresponding to human Abi-1 phosphorylated at Ser225.

アプリケーション

Research Category
細胞骨格

Research Sub Category
細胞骨格シグナル伝達

This phospho-Abi-1 antibody is validated for use in WB for the detection of the phospho-Abi-1 protein.

Western Blot Analysis: A representative lot was used by an independent laboratory in human mammary cells treated with EGF and Abi-1-transfected 293T cells (Mendoza, M., et al. (2011). Molecular Cell 41:661–671).

品質

Evaluated by Western Blot in EGF-treated COS cells untreated and treated with lambda phosphatase.

Western Blot Analysis: 1 µg/mL of this antibody detected Abi-1 in 10 µg of EGF-treated COS cells untreated and treated with lambda phosphatase.

ターゲットの説明

~60 kDa observed.
55 kDa calculated.

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
EGF-treated COS cells untreated and treated with lambda phosphatase.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable