07-2119

Anti-phospho-RalA Antibody (Ser194)

from rabbit, purified by affinity chromatography

• 別名: Ras-related protein Ral-A
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• クローン: polyclonal
• application: WB; inhibition assay
• 化学種の反応性: human
• テクニック: inhibition assay: suitable (peptide); western blot: suitable

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: rat (based on 100% sequence homology), zebrafish (based on 100% sequence homology), bovine (based on 100% sequence homology), Xenopus (based on 100% sequence homology)
• テクニック: inhibition assay: suitable (peptide); western blot: suitable
• NCBIアクセッション番号: NP_005393
• UniProtアクセッション番号: P11233
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: phosphorylation (pSer194)
• 遺伝子情報: human ... RALA(5898)

詳細

詳細

RalA and RalB are Ras-family small monomeric GTPases that can be activated through a Ras-dependent mechanism. The two are highly homologous (89%). Ral proteins are proximal effectors of oncogenic Ras and their activity is needed to support tumorigenesis in certain cancers. Mutants of Ras that are defective in activating all known Ras effectors except Ral GEF are still able to induce invasive phenotypes in transfected cells, suggesting a role for Ral in these processes. RalA is required for anchorage-independent proliferation and tumorigeneisis by driving exocyst assembly and polarized exocytosis in epithelial cells.

特異性

This antibody recognizes RalA phosphorylated at Ser194.

免疫原

KLH-conjugated linear peptide corresponding to human RalA phosphorylated at Ser194.

アプリケーション

Detect the phospho-RalA (Ser194) protein using this Anti-phospho-RalA (Ser194) validated for use in WB & Peptide Inhibition Assay.

Western Blot Analysis: A representative lot detected phospho-RalA (Ser194) in certain primary and metastatic cell lysates, and in certain unmatched tumor tissue lysate samples.

品質

Evaluated by Western Blot in PANC10.05 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected phospho-RalA in PANC10.05 cell lysate. Peptide inhibition was observed with the addition of phospho peptides but not with the addition of non-phospho peptides.

ターゲットの説明

~25 kDa observed. Uncharacterized bands may be observed at ~20 kDa, 100 kDa, and 130-170 kDa in some cell lysates.

アナリシスノート

Control
PANC10.05 cell lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable