07-1285

Anti-acetylated-Src (Lys430) Antibody

from rabbit, purified by affinity chromatography

• 別名: SRC
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: polyclonal
• application: WB; cell based assay
• 化学種の反応性: human
• テクニック: cell based assay: suitable; western blot: suitable

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
• テクニック: cell based assay: suitable; western blot: suitable
• NCBIアクセッション番号: NP_005408
• UniProtアクセッション番号: P12931
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: acetylation (Lys430)
• 遺伝子情報: human ... SRC(6714)

詳細

詳細

Src is a proto-oncogenic tyrosine kinase belonging to the Src family of non-receptor tyrosine kinases. This proto-oncogene plays a pivotal role cellular processes such as proliferation, migration, transformation and regulation of embyronic development and cell growth. Src tyrosine kinases transmit integrin-dependent signals for cell movement and proliferation. The study of Src proteins have contributed to the understanding of cancer caused by abnormal cellular signaling.

特異性

This antibody recognizes Src acetylated at Lys430.

免疫原

Epitope: Acetylated Lys430

KLH-conjugated linear peptide corresponding to human Src acetylated at Lys430.

アプリケーション

Research Category
細胞骨格

Research Sub Category
Cytoskeleton

This Anti-acetylated-Src (Lys430) Antibody is validated for use in WB, Cell Function Assay for the detection of acetylated-Src (Lys430).

Western Blot Analysis: 5 µg/mL from a representative lot detected Src on 10 µg of HEK293T cells transfected with myc-tagged Src and CBP.

品質

Western Blot/Peptide Inhibition Assay Analysis: This antibody peptide blocked on HEK293T cells transfected with myc-tagged Src and CBP.

ターゲットの説明

~69 kDa observed.
Endogenous Src has a molecular weight of ~60 kDa; however, the myc tag adds ~9 kDa to the weight of the protein.

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
HEK293T cells transfected with myc-tagged Src and CBP

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids