07-013

Anti-MeCP2 Antibody

Upstate^®, from rabbit

• 別名: MeCP-2 protein, mental retardation, X-linked 16, mental retardation, X-linked 79, methyl CpG binding protein 2, methyl CpG binding protein 2 (Rett syndrome)
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: rat, mouse
• 包装: antibody small pack of 25 μg
• メーカー／製品名: Upstate^®
• テクニック: western blot: suitable
• アイソタイプ: IgG
• NCBIアクセッション番号: NP_004983
• UniProtアクセッション番号: P51608
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Mecp2(17257) ; rat ... Mecp2(29386)

詳細

詳細

NA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA and repress transcription both directly and by association with known corepressor proteins which include members of the histone deacetylase protein families.

特異性

Predicted to cross-react with human based on the conservation of the immunogenic sequence.

Recognizes MeCP2, Mr 75 kDa. A protein at ~75 kDa was also observed in HeLa nuclear extract. This protein was competed by pre-incubation of the antibody with the immunogenic peptide, suggesting that some HeLa strains contain MeCP2 or a related protein, contrary to a previous report.

免疫原

KLH-conjugated, synthetic peptide corresponding to amino acids 465-478 of mouse MeCP2 (C-PRPNREEPVDSRTP) with an N-terminal cysteine added for conjugation purposes.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
ヒストン

Use Anti-MeCP2 Antibody (rabbit polyclonal antibody) validated in WB to detect MeCP2 also known as MeCP-2 protein, mental retardation X-linked 16, mental retardation X-linked 79, methyl CpG binding protein 2.

品質

Evaluated by Western Blot on Mouse Brain lysates.

ターゲットの説明

~75 kDa observed

物理的形状

Protein A purified

Format: Purified

Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Mouse Brain lysate.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.