06-942-AF647

Anti-acetyl-Histone H3 (Lys9) Antibody, Alexa Fluor^™ 647 Conjugate

from rabbit, ALEXA FLUOR^™ 647

• 別名: Lys9-acetylated
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 結合体: ALEXA FLUOR^™ 647
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: rat, mouse, human
• テクニック: flow cytometry: suitable; immunocytochemistry: suitable
• NCBIアクセッション番号: NP_003520
• UniProtアクセッション番号: Q16695
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: acetylation (Lys9)
• 遺伝子情報: human ... HIST1H3F(8968)

詳細

詳細

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.

特異性

Histone H3 acetylated at position 9.

免疫原

KLH-conjugated synthetic peptide (ARTKQTARK(Ac)STG-C) corresponding to amino acids 1-12 of Histone H3 with acetylated Lys9.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Anti-acetyl-Histone H3 (Lys9), Alexa Fluor^™ 647 Conjugate Antibody, Cat. No. 06-942-AF647, is a highly specific rabbit polyclonal antibody that targets histone H3 Lys9 acetylation (H3K9ac) and has been tested in Flow Cytometry and Immunocytochemistry.

Flow Cytometry Analysis: 2 µL from a representative lot detected cell population with histone H3 Lys9 acetylation (H3K9ac) among one million HEK293 cells.

The unconjugated antibody (Cat. No. 06-492) is shown to be suitable also for Western blotting, dot blot, flow cytometry, chromatin immunoprecipitation (ChIP) and ChIP-seq applications.

品質

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected histone H3 Lys9 acetylation (H3K9ac) in HeLa cells.

ターゲットの説明

~17 kDa observed.

物理的形状

Protein A purified.

Purified rabbit polyclonal antibody in PBS with 1.5% BSA with 0.05% sodium azide

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

法的情報

ALEXA FLUOR is a trademark of Life Technologies

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable