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由来生物
mouse
品質水準
抗体製品の状態
culture supernatant
抗体製品タイプ
primary antibodies
クローン
BU-1, monoclonal
化学種の反応性（ホモロジーによる予測）
all
メーカー／製品名
Upstate®
テクニック
immunocytochemistry: suitable
アイソタイプ
IgG2a
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
詳細
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
特異性
5-Bromo-2-deoxyuridine
免疫原
5-iodouridine:ovalbumin conjugate
アプリケーション
Detect BrdU also known as Bromodeoxyuridine with Anti-BrdU Antibody, clone BU-1 (Mouse Monoclonal Antibody), that has been demonstrated to work in ICC.
品質
A representative lot was evaluated by immunocytochemistry on HeLa cells. A 1:2-1:4 dilution from a representative lot showed positive immunostaining for Anti-BrdU in HeLa cells fixed with 95% ethanol, 5% glacial acetic acid. This lot was tested using the DNA Replication Assay Kit (Catalog # 17-316).
Technical Tip: This antibody detects 5-Bromo-2- deoxyuridine incorporated into double-stranded DNA. DNA denaturation is not required.
Technical Tip: This antibody detects 5-Bromo-2- deoxyuridine incorporated into double-stranded DNA. DNA denaturation is not required.
ターゲットの説明
none given
物理的形状
4 vials, each vial containing mouse culture supernatant lyophilized from 2.5 mL. The supernatant contains mycoplasma, which contribute enzymes that facilitate antibody binding. Lyophilized powder.
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類コード
11 - Combustible Solids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Immunofluorescence labeling indices in myeloma and related monoclonal gammopathies.
Greipp, P R, et al.
Mayo Clinic Proceedings. Mayo Clinic, 62, 969-977 (1987)
X Gao et al.
Cell death & disease, 6, e1610-e1610 (2015-01-23)
G-quadruplex (G4) DNA and G4 DNA resolvase are involved in a variety of biological processes. To understand the biological function of G4 DNA structures and their resolvases in spermatogenesis, we investigated the distribution of G4 structures in mouse testis and
Models of S-phase determination in lymphomas from flow cytometric DNA content histograms: comparison with the bromodeoxyuridine labeling index.
Beauregard, P, et al.
Hematologic Pathology, 5, 177-183 (1991)
Macrophages and dendritic cells as actors in the immune reaction of classical Hodgkin lymphoma.
Tudor, CS; Bruns, H; Daniel, C; Distel, LV; Hartmann, A; Gerbitz, A; Buettner, MJ
Testing null
Difference between random and imprinted X inactivation in common voles.
Elena V Dementyeva,Alexander I Shevchenko,Olga V Anopriyenko,Nina A Mazurok et al.
Chromosoma null
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