05-598
Anti-phospho-Histone H3 (Ser10) Antibody, clone RR002
clone RR002, Upstate®, from mouse
別名:
H3 histone, family 3A, H3S10P, Histone H3 (phospho S10), H3 histone, family 3A, H3 histone, family 3B, H3 histone, family 3B (H3.3B)
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すべての画像(1)
About This Item
UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
RR002, monoclonal
化学種の反応性
vertebrates, nematode, Caenorhabditis elegans, human
メーカー／製品名
Upstate®
テクニック
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
アイソタイプ
IgG
適合性
not suitable for ELISA applications
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
phosphorylation (pSer10)
遺伝子情報
human ... HIST1H3F(8968)
詳細
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
特異性
Broad species cross-reactivity expected due to sequence conservation.
This antibody recognizes Histone H3 phosphorylated at Serine 10.
免疫原
Peptide (ARK[pS]TGGKAPRKQL-C) corresponding to amino acids 7-20 of human histone H3 with a c-terminal cyteine added for conjugation purposes.
アプリケーション
Anti-phospho-Histone H3 (Ser10) Antibody, clone RR002 is a Mouse Monoclonal Antibody for detection of Histone H3 phosphorylated at serine 10. This purified mAb, also known as Anti-H3S10p, is published in peer reviewed journals and is validated in ICC, IHC, WB.
ELISA Analysis:
This antibody showed no immuno-reactivity with an unphosphorylated Histone H3 peptide (residues 7-20), nor with other histone phosphopeptides.
Immunohistochemistry:
A 1:1000 dilution of this antibody detected mitotic cells in C. elegans embryo sections as shown by an independent laboratory.
Immunocytochemistry:
This lot showed positive staining of mitotic cells in cultures of HeLa cells undergoing exponential growth
This antibody showed no immuno-reactivity with an unphosphorylated Histone H3 peptide (residues 7-20), nor with other histone phosphopeptides.
Immunohistochemistry:
A 1:1000 dilution of this antibody detected mitotic cells in C. elegans embryo sections as shown by an independent laboratory.
Immunocytochemistry:
This lot showed positive staining of mitotic cells in cultures of HeLa cells undergoing exponential growth
品質
Routinely evaluated by western blot on acid extracted proteins from colcemid-arrested HeLa cells.
Western Blot Analysis:
0.005-0.025 μg/mL of this lot detected phosphorylated Histone H3 in acid extracted proteins from colcemid-arrested HeLa cells.
Western Blot Analysis:
0.005-0.025 μg/mL of this lot detected phosphorylated Histone H3 in acid extracted proteins from colcemid-arrested HeLa cells.
ターゲットの説明
17 kDa
物理的形状
Format: Purified
Purified mouse IgG in buffer containing 0.1M Tris Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.
アナリシスノート
Control
UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue, HEPG2 cell extrats.
UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue, HEPG2 cell extrats.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類コード
10 - Combustible liquids
WGK
WGK 1
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Protein phosphatase 2A activity affects histone H3 phosphorylation and transcription in Drosophila melanogaster
Nowak, S. J., et al
Molecular and cellular biology, 23, 6129-6138 (2003)
Functional consequences of cyclin D1/BRCA1 interaction in breast cancer cells.
Kehn, K; Berro, R; Alhaj, A; Bottazzi, ME; Yeh, WI; Klase, Z; Van Duyne, R; Fu, S; Kashanchi, F
Oncogene null
Activating RNAs associate with Mediator to enhance chromatin architecture and transcription.
Lai, F; Orom, UA; Cesaroni, M; Beringer, M; Taatjes, DJ; Blobel, GA; Shiekhattar, R
Nature null
Dany S Adams et al.
Development (Cambridge, England), 134(7), 1323-1335 (2007-03-03)
In many systems, ion flows and long-term endogenous voltage gradients regulate patterning events, but molecular details remain mysterious. To establish a mechanistic link between biophysical events and regeneration, we investigated the role of ion transport during Xenopus tail regeneration. We
A role for Caenorhabditis elegans chromatin-associated protein HIM-17 in the proliferation vs. meiotic entry decision.
Bessler, JB; Reddy, KC; Hayashi, M; Hodgkin, J; Villeneuve, AM
Genetics null
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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