05-1431

Anti-Casein Kinase 2α Antibody, 1AD9

clone 1AD9, from mouse

• 別名: CK2 catalytic subunit alpha, CKII alpha, Casein kinase II subunit alpha, Casein kinase 2, alpha 1 polypeptide, Casein kinase II alpha 1 subunit, Casein kinase II-alpha subunit, Protein kinase CK2
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 1AD9, monoclonal
• 化学種の反応性: human, mouse, rat
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG1
• NCBIアクセッション番号: NM_001895.3
• UniProtアクセッション番号: P68400
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CSNK2A1(1457)

詳細

詳細

Unlike most serine/threonine kinases, Casein Kinases (CKs) are not activated by specific stimuli, but are essentially constitutively active. CK1 autophosphorylates, causing inactivation, and protein phosphatases reactivate the kinase. The substrates of CKs are numerous, but their function is less clear, perhaps due to the absence of obvious regulation. CK1 isoforms are thought to be involved in the response to DNA damage, while CK2 levels are elevated in many types of cancer.

特異性

The antibody binds specifically to the catalytic α-subunit of casein kinase2 (CK-2α). The antibody immunoprecipitates a protein with a molecular weight of 44 kD, CK-2α subunit. A 26 kD protein band, the β-subunit of the heterotetrameric holoenzyme α2β2, is coprecipitated with the CK-2α subunit. In western blots with extracts of kidney tissue, the antibody detects various protein bands between 40 and 44 kDa. Using 1 μg/mL of antibody, the lower detection limit for the holoenzyme is 500 ng in Western blot and 100 ng in dot blot; the detection limits for the α-subunit are 300 ng and 60 ng, respectively.

免疫原

Epitope: Region common to both alpha and alpha′ subunits

Purified recombinant α-subunit of casein kinase 2.

アプリケーション

Research Category
細胞シグナル伝達

アポトーシス及び癌

Research Sub Category
発生シグナル伝達

Anti-Casein Kinase 2α Antibody, 1AD9 detects level of Casein Kinase 2α & has been published & validated for use in IC, WB, IH(P) & IP.

Immunoprecipitation: Recommended

Immunocytochemistry: A previous lot of this antibody was used at a dilution of 5 μg/mL (acetone or methanol fix only).
Optimal working dilutions must be determined by end user.

Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of Casein Kinase 2 Monoclonal Antibody: S-KB-3 Cells

品質

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected Neurofilament on 10 μg of PC12 lysates.

ターゲットの説明

44 kDa

関連事項

Replaces: 04-1129

物理的形状

Purified

Format: Purified

Purified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate, pH 7.6 with 0.25 M NaCl and 0.1% sodium azide.

保管および安定性

Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

アナリシスノート

Control
HeLa cell lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable