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由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
3E4.2, monoclonal
化学種の反応性
human
メーカー／製品名
Upstate®
テクニック
western blot: suitable
入力
sample type mesenchymal stem cell(s)
アイソタイプ
IgG1κ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... TBXT(6862)
詳細
The Brachyury protein is a T-box transcription factor that is one of the earliest indicators of mesoderm formation during embryonic development. As the brachyury protein family is essential for the formation of the posterior body in all vertebrates, Brachyury has been shown to be expressed during gastrula and neurula stages, as well as in the developing notochord. Consequently, when mutations occur within the brachyury gene, severe vertebral malformations have been found to occur, as a direct result. In addition, current findings also indicate that the dysregulation of brachyury may also be linked to the formation of chordomas, which are malignant tumors that develop along the spine.
特異性
Reactivity with other species has not been tested.
This monoclonal antibody recognizes the brachyury protein.
免疫原
Epitope: C-terminus
Synthetic human brachyury peptide, a.a. 224-435.
アプリケーション
Research Category
幹細胞研究
幹細胞研究
Research Sub Category
間葉幹細胞
間葉幹細胞
This Anti-Brachyury Antibody, clone 3E4.2 is validated for use in WB for the detection of Brachyury.
品質
Routinely evaluated by Western blot on NT2 cell lysates.
Western Blotting:
Recommended working dilutions are 1:1000.
Western Blotting:
Recommended working dilutions are 1:1000.
ターゲットの説明
47 kDa
物理的形状
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.
保管および安定性
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
04-135:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Brachyury, a crucial regulator of notochordal development, is a novel biomarker for chordomas.
Vujovic, S, et al.
The Journal of Pathology, 209, 157-165 (2006)
Chromatin remodeling during mouse and human embryonic stem cell differentiation.
Golob, Jonathan L, et al.
Developmental Dynamics, 237, 1389-1398 (2008)
T P Yamaguchi et al.
Genes & development, 13(24), 3185-3190 (2000-01-05)
Wnt3a encodes a signal that is expressed in the primitive streak of the gastrulating mouse embryo and is required for paraxial mesoderm development. In its absence cells adopt ectopic neural fates. Embryos lacking the T-box-containing transcription factors, Brachyury or Tbx6
Development of antibodies to human embryonic stem cell antigens.
Cai, Jingli, et al.
BMC Developmental Biology, 5, 26-26 (2005)
Hematopoiesis from human embryonic stem cells: overcoming the immune barrier in stem cell therapies.
Priddle, Helen, et al.
Stem Cells, 24, 815-824 (2006)
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