アッセイ
≥95%
形状
powder
保管温度
−20°C
アプリケーション
Enantioprobe (S)-4 is one of 16 fully functionalized, enantiomeric fragment probes developed in the Cravatt lab. Combining fragment-based ligand discovery (FBLD) with chemical proteomics, the enantioprobe library assesses ligandability across proteomes. Each enantioprobe contains a structurally variable fragment for interaction with proteins, photoactivatable diazirine for UV-induced covalent protein labeling, and bioorthogonal alkyne handle for detection,
enrichment, and identification. Of the eight enantiomeric pairs, each differs by one stereocenter, and comparing stereoselective fragment-protein interactions between the pairs simplifies validation of authentic protein-binding events. Enantioprobe (S)-4′s paired fragment is available as Enantioprobe (R)-4 (cat# 912913).
Together, the 16 enantioprobes support ligandability studies in living cells, a significant method for development of chemical probes and lead discovery efforts to find binders for traditionally "undruggable" protein targets. This strategy is also compatible with multiplexing for higher throughput.
enrichment, and identification. Of the eight enantiomeric pairs, each differs by one stereocenter, and comparing stereoselective fragment-protein interactions between the pairs simplifies validation of authentic protein-binding events. Enantioprobe (S)-4′s paired fragment is available as Enantioprobe (R)-4 (cat# 912913).
Together, the 16 enantioprobes support ligandability studies in living cells, a significant method for development of chemical probes and lead discovery efforts to find binders for traditionally "undruggable" protein targets. This strategy is also compatible with multiplexing for higher throughput.
Supporting reagents:
- Fluorophore-conjugated azide tags for SDS-PAGE in-gel profiling: 910147, 760757, 760765, https://www.sigmaaldrich.com/catalog/product/aldrich/777315?lang=en®ion=US, 777323
- Azide-biotin tags for enrichment: 901765, 914134, 914460, 762024, 900912, 900891, QBD10825)
- Azide capture solid supports: 900957, 742627
- Streptavidin for biotin enrichment: S6940, E5529, E2513, 69203, 16-126, S1638
関連製品
製品番号
詳細
価格
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
913189-5MG:
913189-BULK:
913189-50MG:
913189-VAR:
最新バージョンのいずれかを選択してください：
資料
Ligandability describes the propensity of a protein target to bind a small molecule with high affinity. It is a precursor to evaluating druggability, which requires more advanced translational pharmacological effects and drug-like properties in vivo.
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