ZRB1452

Anti-MyoD Antibody, clone 1F22 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Class C basic helix-loop-helix protein 1, Myf-3, Myoblast determination protein 1, Myogenic factor 3, bHLHc1
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit (recombinant)
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1F22, monoclonal; recombinant monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 34.5 kDa; observed mol wt 34 kDa
• species reactivity: human, mouse
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• UniProt accession no.: P15172
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... MYOD1(4654)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone IF22 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Myoblast determination protein 1 (MyoD1). It targets an epitope within the C-terminal half.

Immunogen

His-tagged recombinant protein fragment corresponding to 166 amino acids from the C-terminal half of human Myoblast determination protein 1 (MyoD1).

Application

Anti-MyoD, clone 1F22 ZooMAb, Cat. No. ZRB1452, is a recombinant Rabbit monoclonal antibody that specifically targets MyoD and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected MyoD in A431, AC16, C2C12, HeLa, HepG2, NIH 3T3 cells.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MyoD in human alveolar rhabdomyosarcoma tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound recombinant MyoD protein fragment with a KD of 1.3 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Myoblast determination protein 1 (UniProt: P15172; also known as Class C basic helix-loop-helix protein 1, bHLHc1, Myogenic factor 3, Myf-3) is encoded by the MYOD1 (also known as BHLHC1, MYF3, MYOD) gene (Gene ID: 4654) in human. MyoD is a skeletal muscle-specific bHLH transcription factor that is activated during myogenesis and commits undifferentiated cells to the muscle lineage. It is a master regulator of the muscle-specific transcription program and it controls the expression of many structural, functional, and metabolic skeletal muscle-specific genes. It is also involved in muscle repair process. MyoD forms heterodimers with the bHLH E-proteins E12 and E47 and activates transcription through binding to the CACCTG/CAGCTG E-box sequences. It can undergo phosphorylation by CDK9, and this phosphorylation promotes its function in muscle differentiation. MyoD is shown to interact with and is inhibited by the Twist protein. MYOD gene is reported to be a clock-controlled gene whose rhythmic expression is driven by the activity of CLOCK:BMAL1 heterodimer. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1