Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

Safety Information

MAB3878

Sigma-Aldrich

Anti-MyoD1 Antibody

clone 5.2F, Chemicon®, from mouse

Synonym(s):

Anti-MYF3, Anti-MYOD, Anti-MYODRIF, Anti-PUM, Anti-bHLHc1

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5.2F, monoclonal

species reactivity

rat, mouse, chicken, human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2a

NCBI accession no.

NM_002478.4

UniProt accession no.

P15172

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MYOD1(4654)

Specificity

Recognizes MyoD1. The epitope is localized to amino acids 3-56 in the N-terminus of mouse MyoD1 protein.

Immunogen

Recombinant protein from mouse MyoD1.

Application

Anti-MyoD1 Antibody is a high quality Mouse Monoclonal Antibody for the detection of MyoD1 & has been validated in ICC, IHC, IP & WB.
Western blot: 1 μg/mL. Reacts with the ~45 kD protein.

Immunohistochemistry on frozen and formalin-fixed, paraffin embedded tissue: 2-4 μg/mL.

Immunocytochemistry

Immunoprecipitation: 2 μg/mg of protein lysate

Optimal working dilutions must be determined by end user.

Target description

~ 45 kDa

Physical form

Format: Purified

Analysis Note

Control
Fetal Muscle, Rhabdomyosarcoma

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Not finding the right product?  

Try our Product Selector Tool.

recommended

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

MAB3878:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

A recurrent neomorphic mutation in MYOD1 defines a clinically aggressive subset of embryonal rhabdomyosarcoma associated with PI3K-AKT pathway mutations.
Kohsaka, S; Shukla, N; Ameur, N; Ito, T; Ng, CK; Wang, L; Lim, D; Marchetti, A; Viale et al.
Nature Genetics null
Prenatal and early postnatal exposure to high-saturated-fat diet represses Wnt signaling and myogenic genes in offspring rats.
Ke-Feng Yang,Xiu-Hua Shen,Wei Cai
Experimental Biology and Medicine (Maywood, N.J.) null
L V Renna et al.
European journal of histochemistry : EJH, 58(4), 2444-2444 (2015-01-13)
Myotonic dystrophy type 1 (DM1) and type 2 (DM2) are multisystemic disorders linked to two different genetic loci and characterized by several features including myotonia, muscle weakness and atrophy, cardiac dysfunctions, cataracts and insulin-resistance. In both forms, expanded nucleotide sequences
In situ mechanotransduction via vinculin regulates stem cell differentiation.
Holle, AW; Tang, X; Vijayraghavan, D; Vincent, LG; Fuhrmann, A; Choi, YS; del Alamo, JC; Engler, AJ
Stem Cells null
Junmin Lee et al.
Biomaterials, 69, 174-183 (2015-08-19)
Cells sense and transduce the chemical and mechanical properties of their microenvironment through cell surface integrin receptors. Traction stress exerted by cells on the extracellular matrix mediates focal adhesion stabilization and regulation of the cytoskeleton for directing biological activity. Understanding

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service