ZRB1158
Anti-VGAT Antibody, clone 2E6 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
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About This Item
UNSPSC Code:
12352203
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2E6, monoclonal
recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt ~57.42 kDa
observed mol wt ~64 kDa
species reactivity
human
species reactivity (predicted by homology)
mouse, rat, monkey, bovine
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 2E6 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects vesicular GABA transporter. It targets an epitope within 15 amino acids from the C-terminal, cytoplasmic domain.
Immunogen
KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal, cytoplasmic domain of human Vesicular GABA transporter.
Application
Quality Control Testing
Evaluated by Western Blotting in Human Brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected VGAT in Human Brain tissue lysate.
Tested Applications
Immunocytochemistry Analysis: A 1:100 Dilution from a representative lot detected VGAT in Y79 cell line.
Affinity Binding Assay Analysis: A representative lot of this antibody bound VGAT peptides with a KD of 5.3 x 10^-8 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected VGAT in human cerebellum tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Human Brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected VGAT in Human Brain tissue lysate.
Tested Applications
Immunocytochemistry Analysis: A 1:100 Dilution from a representative lot detected VGAT in Y79 cell line.
Affinity Binding Assay Analysis: A representative lot of this antibody bound VGAT peptides with a KD of 5.3 x 10^-8 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected VGAT in human cerebellum tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-VGAT, clone 2E6 ZooMAb®, Cat. No. ZRB1158, is a recombinant Rabbit monoclonal antibody that specifically targets VGAT and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Target description
Vesicular inhibitory amino acid transporter (UniProt: Q9H598; also known as GABA and glycine transporter, Solute carrier family 32 member 1, Vesicular GABA transporter, hVIAAT, VGAT) is encoded by the SLC32A1 (also known as VGAT, VIAAT) gene (Gene ID: 140679) in human. VGAT is a multi-pass membrane protein with six cytoplasmic domains, ten transmembrane domains, and five vesicular lumenal domains. It is shown to be highly concentrated in the nerve endings of GABAergic neurons and in glycinergic nerve endings. It transports GABA and glycine into acidic vesicles and localizes to the synaptic vesicle in glycinergic and GABAergic neurons. It is also expressed throughout the horizontal cell tips at photoreceptor terminals in retina. VGAT expression has been reported in pancreatic islets of rat, however, its expression in human pancreatic islets is none to very low. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Chessler, SD., et al. (2002). Diabetes. 51(6); 1763-1771).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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