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Safety Information

AB5062P

Sigma-Aldrich

Anti-Vesicular GABA Transporter (VGAT) Antibody

Chemicon®, from rabbit

Synonym(s):

Vesicular Inhibitory Amino Acid Transporter

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, monkey, human, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NM_080552.2

UniProt accession no.

Q9H598

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... SLC32A1(140679)

General description

Vesicular GABA Transporter (unc-47) homologue is a 525 amino acid long protein belonging to the family of neurotransmitter transporter (1). VGAT functions in the uptake of GABA and glycine into synaptic vesicles. Since the protein is bound to synaptic vesicles, VGAT antibodies are excellent markers of presynaptic GABAergic and glycinergic neurons.

Specificity

Antibody cross-reactivity with various species is not known. The immunogen sequence is 100% conserved in mouse, human and monkey.
Specific for Vesicular GABA Transporter (VGAT). Vesicular GABA Transporter (unc-47) homologue is a 525 amino acid long protein belonging to the family of neurotransmitter transporter. The immunogen peptide shows no significant homology with other known proteins.

Immunogen

A 17 amino acid peptide sequence near the carboxy terminal region of rat VGAT (McIntire, S., 1997).

Application

Immunohistochemistry/Immunofluorescence:
2-20 µg/mL of a previous lot was used on paraformaldehyde fixed sections.

ELISA:
1:10,000 – 1:100,000 dilution of a previous lot was used on ELISA, using 50-100 ng of control peptide (Cat. No. AG379) per well.

Western blot:
1-10 µg/mL (Chemiluminescence technique) The expected molecular weight is approximately 55-60 kDa.

Optimal working dilutions must be determined by the end user.
This Anti-Vesicular GABA Transporter (VGAT) Antibody is validated for use in ELISA, IF, IH, WB for the detection of Vesicular GABA Transporter (VGAT).

Quality

Evaluated by Western Blot on Rat retina lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected Vesicular GABA Transporter on 10μg of rat retina lysates.

Target description

57 kDa

Physical form

Purified rabbit polyclonal in buffer containing PBS with 0.1% BSA.

Analysis Note

Control
Brain tissue, rat retina lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

AB5062P:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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R Gómez-Nieto et al.
Neuroscience, 154(1), 51-64 (2008-04-04)
Afferents to the primary startle circuit are essential for the elicitation and modulation of the acoustic startle reflex (ASR). In the rat, cochlear root neurons (CRNs) comprise the first component of the acoustic startle circuit and play a crucial role
GABAergic neurons intermingled with orexin and MCH neurons in the lateral hypothalamus discharge maximally during sleep.
Oum Kaltoum Hassani,Pablo Henny,Maan Gee Lee,Barbara E Jones
The European Journal of Neuroscience null
Characterization of axo-axonic synapses in the piriform cortex of Mus musculus.
Xinjun Wang,Qian-Quan Sun
The Journal of Comparative Neurology null
Cleavage of the vesicular GABA transporter under excitotoxic conditions is followed by accumulation of the truncated transporter in nonsynaptic sites.
Gomes, Jo?o R, et al.
The Journal of Neuroscience, 31, 4622-4635 (2011)
Phillip R Kramer et al.
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