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Key Documents

Safety Information

T9568

Sigma-Aldrich

Trizma® Pre-set crystals

BioPerformance Certified, pH 8.9, average Mw 125.6

Synonym(s):

Tris crystals

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

grade

BioPerformance Certified

form

crystalline powder

mol wt

average Mw 125.6

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, NICKase, none detected
 endotoxin, tested
 total aerobic microbial count, tested
≤0.05 A290
≤0.5% water (Karl Fischer)
≤5 ppm Heavy metals (as Pb)

pH

8.90 (25 °C, 0.1 M)(lit.)
8.9

useful pH range

7.0-9.0

solubility

water: 0.66 g/mL, clear, colorless

application(s)

diagnostic assay manufacturing

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Application

Dissolve in water to make a 0.1M solution. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.
The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.

Other Notes

This product is a pre-mixed combination of Trizma base and Trizma HCl that can be used to prepare a common pH value for Tris buffer.
Certain pH electrodes do not give accurate results with Tris buffers.

Preparation Note

Trizma solutions can be autoclaved.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

T9568-250G:
T9568-100G:
T9568-VAR:
T9568-BULK-PC:
T9568-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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