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Biological Buffers

Image of light blue biological buffer solution in beaker with pipette.

It’s important to protect the integrity of your biomolecules and reagents by choosing the right biological buffer systems. We offer an extensive portfolio of biological buffers for a wide variety of applications, including cell culture buffers, PCR buffers, HEPES buffers, and assay buffers.

Key features of our advanced buffer systems:

  • High-purity solutions available in a wide range of grades
  • Multiple and user-friendly packaging options
  • Assured solution stability with pH control
  • No interference with biological processes
  • Add critical salts and nutrients to cells and tissues
  • Scalable from early research to commercial applications
  • Customized blending

Our advanced buffering systems can bring phenomenal stability to your cell culture, polymerase chain reaction (PCR), drug screening, bioprocessing, purification, and final formulation-based applications. All our products can be scaled from early research to commercial applications.

Products

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Product Number
Product Description
Pricing
D8537
Dulbecco′s Phosphate Buffered Saline, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture
T1503
Trizma® base, Primary Standard and Buffer, ≥99.9% (titration), crystalline
P4417
Phosphate buffered saline, tablet
H0887
HEPES solution, 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture
H4034
HEPES, BioPerformance Certified, ≥99.5% (titration), suitable for cell culture
S5761
Sodium bicarbonate, powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture
R0278
RIPA Buffer
806552
Phosphate Buffered Saline, pH 7.4, liquid, sterile-filtered, suitable for cell culture
T6066
Trizma® base, BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)
252859
Tris(hydroxymethyl)aminomethane, ACS reagent, ≥99.8%
E5134
Ethylenediaminetetraacetic acid disodium salt dihydrate, suitable for electrophoresis, for molecular biology, 99.0-101.0% (titration)
D8662
Dulbecco′s Phosphate Buffered Saline, With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture
S0751
Sodium phosphate monobasic, ReagentPlus®, ≥99.0%
P3563
Phosphate Buffered Saline with 0.05% TWEEN® 20, pH 7.4
93283
Tris-EDTA buffer solution, BioUltra, for molecular biology, pH 8.0
M1254
MOPS, ≥99.5% (titration)
P5655
Potassium phosphate monobasic, powder, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%
P5493
Phosphate buffered saline, 10× concentrate, BioPerformance Certified, suitable for cell culture
S2889
Sodium acetate, anhydrous, for molecular biology, ≥99%
M3671
MES, low moisture content, ≥99% (titration)
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Related Product Resources

Download: Buffer Selection Guide
Flyer: pHast Pack Electrophoresis
Flyer: Tricine Buffer
Flyer: HEPES Buffer
Flyer: Trizma Buffer

Article: Buffer Reference Center
Article: Polymerase Chain Reaction (PCR) Basics
Article: TAE and TBE Running Buffers Recipe & Video
Article: M-Clarityâ„¢ Program
Article: Buffer Calculator

Complete Workflow Solutions

We provide a comprehensive selection of high-quality biochemicals in a wide range of buffer grades and innovative, user-friendly packaging options. In addition to off-the-shelf products, we offer custom reagent manufacturing and customized blending.

Bioperformance Certified Buffers

Our superior BioPerformance Certified buffers are stringently tested and pre-qualified to expedite your bioprocessing workflows in critical life science applications, such as cell culture, molecular biology, and diagnostic manufacturing. This may include enzymatic assay of nuclease, protease, and phosphatase, cellular toxicity, or endotoxin and bioburden levels. Using a single high-quality buffer across multiple workflows ensures the reproducibility of your experiment.

pHast Packâ„¢ Ready-to-Use Buffers

Trying to get the correct concentration and pH value in buffer preparations can be a tedious and time-consuming task. Our pHast Packâ„¢ ready-to-use buffers are specially designed to simplify your work. Simply mix the precisely pre-weighed packets with distilled water for quick and reproducible results again and again. By saving time, effort and even shelf space, you can focus on more important areas of your research endeavors.

Need help making a buffer? Our buffer preparation calculator helps you to quickly calculate buffer molarity, mass, and volume.

Related Product Categories
Molecular Biology & Functional Genomics
Explore our gene editing & functional genomics, molecular biology, oligos & qPCR probes, and immunoassay reagents and resources to make your next discovery.
PCR Reagents & Kits
Polymerase Chain Reaction (PCR) is a mainstay in molecular biology labs. PCR is used for amplifying DNA by several orders of magnitude and our reagents and resources are suitable for numerous research workflows including, gene expression analysis, genotyping, sequencing, and mutagenesis application needs.
Protein Electrophoresis & Western Blotting
Browse our products for protein electrophoresis and Western blotting, including Immobilon transfer membranes, transfer buffers, electrophoresis equipment, gels, gel stains, protein markers, and immunodetection reagents.
Protein Gel Electrophoresis and Transfer Equipment
Electrophoresis tanks, blotting systems, and power supplies for protein gel electrophoresis and wet and semi-dry transfer.
Protein Electrophoresis Gels & Buffers
Precast polyacrylamide protein gels, running buffers, and hand-casting reagents for hand-pouring polyacrylamide gels for PAGE and SDS-PAGE protein gel electrophoresis.
Detergents - Anionic, Cationic, Zwitterionic, Anti-foaming
We offer a broad range of biological detergents and surfactants, including REACH-compliant biodegradable alternatives, for use in cell lysis, DNA/RNA electrophoresis, Western blotting, transfection, and many other life science research applications.
Antibiotics
We offer a comprehensive portfolio of antibiotics to prevent contamination of cell cultures through pathogens or other cell lines, for genetic marker selection and cell biology research.
Agarose: Properties and Research Applications
We offer agarose bioreagents in a selection of grades suitable for cell culture and molecular biology applications ranging from chromatography to nucleic acid & protein gel electrophoreses. The pHast Packâ„¢ ready-to-pour agarose gels are formulated to save time and effort in native RNA and DNA electrophoresis assays.
Related Applications
IVD Manufacturing
A brief overview on the key components involved in successful In vitro diagnostic (IVD) assay development. Learn more on IVD scale up, manufacturing, and commercialization.
PCR Applications
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
Cloning & Expression
Cloning, protein expression vectors and techniques for optimal gene cloning and protein expression systems.
Protein Purification
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
Protein Quantitation
Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.
Western Blotting
An overview of the principles and applications of Western blotting for protein detection. Includes links to detailed protocols, videos, and other resources for protein extraction, gel electrophoresis, transfer to PVDF or nitrocellulose membranes, and chemiluminescent, colorimetric, and fluorescent detection methods.
Gel Electrophoresis
Protein gel electrophoresis is used to separate proteins for purification, characterization, and detection. Methods such as native PAGE, SDS-PAGE, 2D PAGE, and isoelectric focusing (IEF) are used in preparation for downstream applications including Western blotting, mass spectrometry, and proteomic analysis.
Cell Culture Media Preparation
Culture media preparation for in vitro cell cultivation including preparation of different media formats, sterilization methods, cell growth and regulation supplements, media storage and use.
Related Services
Bulk and Custom Services
We offer an extensive, reliable supply of production materials in bench to bulk volumes. Our custom services include quality control, testing, documentation services, chemical packaging, contract manufacturing according to your specifications.

Highlights


HEPES induces lysosomes and autophagy in culture cells
HEPES induces lysosomes & autophagy in culture cells - a call for action
Discover how HEPES may influence cell culture experiments and what to be aware of in data interpretation.



Webinar: Getting Your Buffers Right: How to Control the pH of Cell Culture Medium
This webinar will explain the principles of good practice in controlling the acid-base balance of culture media. It will highlight those combinations of buffers that can result in poor pH control, non-intuitive outcomes and erroneous inferences.

Slide 1 of 2

Buffer Selection Guide
Request your Buffer Selection Guide
The Biological Buffers Selection Guide can be a great reference piece to hang in your lab. Request your DIN A1 (23.4" x 33.1") poster.


pHast Packâ„¢ ready-to-use buffers
Save time, effort, and shelf space with pHast Packâ„¢ ready-to-use buffers. Our formulations are specially developed to simplify your work, and strictly tested to ensure exact concentrations and pH.

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