All Photos(3)

Glycerol

BioUltra, for molecular biology, anhydrous, ≥99.5% (GC)

All Photos(3)

Synonym(s):

1,2,3-Propanetriol, Glycerin

Linear Formula:

HOCH₂CH(OH)CH₂OH

CAS Number:

56-81-5

Molecular Weight:

92.09

Beilstein:

635685

EC Number:

200-289-5

MDL number:

MFCD00004722

PubChem Substance ID:

57651220

NACRES:

NA.25

Quality Level

200

grade

for molecular biology

vapor density

3.1 (vs air)

vapor pressure

<1 mmHg ( 20 °C)

product line

BioUltra

assay

≥99.5% (GC)

form

viscous liquid

autoignition temp.

698 °F

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

ign. residue

≤0.1%

refractive index

n20/D 1.474 (lit.)
n20/D 1.474

pH

5.5-8 (25 °C, 5 M in H₂O)

bp

182 °C/20 mmHg (lit.)

mp

20 °C (lit.)

solubility

H₂O: 5 M at 20 °C, clear, colorless

density

1.25 g/mL (lit.)

anion traces

chloride (Cl^-): ≤1 mg/kg
sulfate (SO₄^2-): ≤10 mg/kg

cation traces

Ag: ≤5 mg/kg
Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
NH₄⁺: ≤5 mg/kg
Na: ≤20 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Tl: ≤5 mg/kg
Zn: ≤1 mg/kg

SMILES string

OCC(O)CO

λ

5 M in H₂O

UV absorption

λ: 260 nm A_max: 0.05
λ: 280 nm A_max: 0.04

InChI

1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2

InChI key

PEDCQBHIVMGVHV-UHFFFAOYSA-N

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General description

Glycerol is odourless, colorless, viscous in nature, and exists as a sweet tasting liquid. It can be derived naturally as well as from petrochemical feedstock. Glycerol has a wide variety of applications, and is one of the most valuable and versatile chemical substances in nature. It can be used as an emollient, solvent, sweetening agent, in pharmaceutical formulations, cosmetics, foodstuffs and toiletries. It is very stable and can be easily stored under normal temperature; also it is non-irritating and has no adverse impact to the environment.

Application

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Packaging

1 L in glass bottle

100, 250 mL in glass bottle

Biochem/physiol Actions

Glycerol is hygroscopic in nature and is soluble in water owing to its three hydrophilic alcoholic hydroxyl groups. It can form both inter- and intramolecular hydrogen bonds, making it a very flexible molecule. The physiologic effect of glycerine is due to cell-mediated immunity, increased IgG production and increased histamine release.

Glycerol has been used as

a supplement during cell culture of Mycobacterium tuberculosis and Mycobacterium avium.
a fuel during designing enzymatic biofuel cell.
a liquid composite matrix with 4-HCCA and 3-aminoquinoline for analysis of neutral and acidic glycans.
a matrix for fast atom bombardment MS.
may be employed as liquid matrix for the quantification studies by MALDI (Matrix-assisted laser desorption/ionization mass spectrometry) analysis.

Other Notes

Component of loading buffer in agarose gel electrophoresis of nucleic acids previously denatured with glyoxal; Preparation of phage and plasmid DNA, for the storage of pure cultures

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

390.2 °F - Pensky-Martens closed cup

Flash Point(C)

199 °C - Pensky-Martens closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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