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T1706

Sigma-Aldrich

TransIT-CRISPR®

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.51

application(s)

CRISPR

shipped in

wet ice

storage temp.

−20°C

General description

Accomplish highly effective transfection of CRISPR/Cas9 DNA, RNA, Cas9-gRNA RNP (ribonucleoprotein) complexes and siRNA to knock-out, modify or knock-down genes of interest in a wide variety of cell types.

Features and Benefits

  • Highly efficient, non-liposomal polymeric transfection/delivery method for use with MISSION CRISPR/Cas9 DNA, RNA, Cas9 RNP Complexes and siRNA.
  • TransIT-CRISPR is a simple, fast, effective, and versatile. One transfection reagent to deliver CRISPR-Cas9 components in a variety of formats.
  • Effective in most cell lines including difficult-to-transfect cells.

Components

0.4ml and 1ml DNA and siRNA non-liposomal, polymeric transfection reagent

Linkage

To order MISSION® CRISPR reagents, please click here

For more information and transfection protocols, please visit SigmaAldrich.com/TransITCRISPR

Other Notes

  • TransIT-CRISPR has been tested in variety of cell types including U20S and HEK 293, A549 and HeLa among others.
  • It is recommended to transfect MISSION CRISPR DNA and RNA with the TransIT-CRISPR transfection reagent.
  • Sigma-Aldrich® is the exclusive distributor of TransIT-CRISPR from Mirus Bio LLC.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMA-ALDRICH is a registered trademark of Merck KGaA, Darmstadt, Germany
TransIT-CRISPR is a registered trademark of Mirus Bio LLC

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225,H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

57.2 °F - closed cup

Flash Point(C)

14.0 °C - closed cup

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

FSL

Group 4: Flammable liquids
Alcohols
Hazardous rank II

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

T1706-VAR:
T1706-BULK:
T1706-1ML:4548174012659
T1706-0.4ML:4548174012666

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
71113920
51053481
51053795
SLBP2593V
SLBP2596V

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Protocols

Ribonucleoprotein (RNP) Protocols using SygRNA ® gRNAs and Cas9 proteins

Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.

Related Content

CRISPR Cas9 Proteins

The largest offering of quality Cas9 proteins ensures you have the perfect CRISPR reagents for your specific experimental needs. Rigorous purification, NLS signals, increased specificity and activity ensure your gene editing experiments are efficient and simple.

Gene Expression & Silencing

Sigma-Aldrich® Advanced Genomics is the leading provider of gene editing and silencing technologies including CRISPR, Cas9, synthetic guide RNA (sgRNA), and Zinc Finger Nuclease (ZFN).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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