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L3287

Escort^™ IV Transfection Reagent

Name: Escort<SUP>™</SUP> IV Transfection Reagent
Brand: SIGMA

Lipid reagent for transient and stable transfection of mammalian and insect cells.

Synonym(s):

Gene delivery

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About This Item

UNSPSC Code:

41106502

NACRES:

NA.25

grade

for molecular biology

Quality Level

100

form

liquid (aqueous solution)

usage

mL sufficient for 160-500 transfections

concentration

1 mg/mL

technique(s)

transfection: suitable

storage temp.

2-8°C

Related Categories

Transfection

General description

Escort^™ IV is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into a wide variety of eukaryotic cell types.

Application

Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 4-16 μL Escort^™ IV and 2 μg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. For a list of cells that have been successfully transfected using Escort^™ IV, see the Transfection Reagent Selection Guide.

Features and Benefits

Suitable for stable and transient transfection
Optimized for a wide variety of cell lines
Low toxicity
Compatible with both serum and serum-free transfection protocols
Ideal for Sf9, Sf21 and S2 insect cells

Components

Escort^™ IV formulation:
1 mg/mL total lipid in water

Note the identity of the lipids used in Escort^™ IV is confidential.

Caution

Do not freeze.

Principle

A stable complex is formed when Escort^™ IV is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.

Legal Information

Escort is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Articles

Introduction to Cell Transfection

Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

Protocols

Lipid Transfection Optimization Protocol

Universal Transfection Reagent Protocol

Sigma's Universal Transfection Reagent is a unique formulation of a proprietary polymer blend used for transient and stable transfection of nucleic acids into various eukaryotic cell lines and hard-to-transfect primary cells. Fast and easy protocol is compatible with serum, serum-free medium and antibiotics

Escort™ IV Transfection Reagent Protocol

Escort^™ IV Transfection Reagent Protocol

Escort™ III Transfection Reagent Protocol

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