Merck
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L3287

Sigma-Aldrich

Escort IV Transfection Reagent

Lipid reagent for transient and stable transfection of mammalian and insect cells.

Synonym(s):
Gene delivery
NACRES:
NA.25

grade

for molecular biology

Quality Level

form

liquid (aqueous solution)

usage

 mL sufficient for 160-500 transfections

concentration

1 mg/mL

technique(s)

transfection: suitable

storage temp.

2-8°C

Related Categories

General description

Escort IV is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into a wide variety of eukaryotic cell types.

Application

Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 4-16 μL Escort IV and 2 μg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. For a list of cells that have been successfully transfected using Escort IV, see the Transfection Reagent Selection Guide.

Features and Benefits

  • Suitable for stable and transient transfection
  • Optimized for a wide variety of cell lines
  • Low toxicity
  • Compatible with both serum and serum-free transfection protocols
  • Ideal for Sf9, Sf21 and S2 insect cells

Components

Escort IV formulation:
1 mg/mL total lipid in water

Note the identity of the lipids used in Escort IV is confidential.

Caution

Do not freeze.

Principle

A stable complex is formed when Escort IV is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.

Legal Information

Escort is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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