SRE0062

Tris-Borate EDTA Buffer, 10X Concentrate

• Synonym(s): TBE Buffer
• UNSPSC Code: 41105319
• NACRES: NA.25

Properties

• form: liquid
• Quality Level: 500
• application(s): diagnostic assay manufacturing
• storage temp.: room temp

Description

Application

Tris-Borate EDTA (TBE) buffer is commonly used in the electrophoresis of nucleic acids in agarose and polyacrylamide gels.

TBE buffer is recommended for resolution of RNA and DNA fragments smaller than 1500 bp.

Physical form

10X TBE buffer stock solution contains 0.89 M Trizma(R) with 0.02 M EDTA adjusted to pH 8.3 with boric acid. Dilute with ultrapure water to 1X.

Notes: If precipitation is present in the 10X concentrated buffer, warm the bottle to 37 °C and mix until completely dissolved prior to dilution.
It is recommended 1X working solutions be filtered through a 0.2 mm filter before use.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H360FD
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Repr. 1B
• Storage Class Code: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable