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Safety Information

PPB010

Sigma-Aldrich

Tris EDTA Buffer, pH 8.0

pHast Pack™, powder, 10X concentrate

Synonym(s):

10X TE buffer

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

product line

pHast Pack™

form

powder

storage condition

(Tightly closed. Dry)

impurities

≤5 ppm Heavy Metals (lead)

color

white

pH

7.9—8.1

solubility

soluble, clear, colorless

cation traces

Fe: <10 ppm

foreign activity

DNAse, none detected
Nickase, none detected
Protease, none detected
RNAse, none detected

storage temp.

room temp

General description

10X Tris-EDTA or TE buffer has two components. Tris buffer is a commonly used stable, non-toxic, and non-hygroscopic buffer. Also, ethylenediaminetetraacetic acid (EDTA), a metal chelator, binds with cation metal ions that further inactivate the DNase enzyme which requires metal ions to activate. It has a pH range of 7.9 - 8.1.

Application

TE buffer is intended for use in:
  • Nucleic acid gel electrophoresis
  • storage and dilution of DNA and RNA
  • DNA automated sequencing
  • Immunohistochemistry

Features and Benefits

  • Fast and easy Ready-to-use buffer
  • Minimizes effort - no need to weigh and adjust pH
  • Saves time and eliminates exposure to hazardous chemicals
  • Biological tests: free of DNase, RNase, protease, and nickase
  • Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Packaging

Foil pouch

Reconstitution

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 10X solution containing 100mM Tris, 10mM EDTA, pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

Storage and Stability

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Health hazard
GHS08

Signal Word

Warning

Hazard Statements

H373

Precautionary Statements

P260 - P314 - P501

Hazard Classifications

STOT RE 2 Inhalation

Target Organs

Respiratory Tract

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

PPB010-20PAK-PW:
PPB010-VAR:
PPB010-BULK:
PPB010-20PAK:

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Certificates of Analysis (COA)

Lot/Batch Number
MKCK2952

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