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574793

Sigma-Aldrich

TE Buffer, 100X, Molecular Biology Grade

A 100X concentrate that, when diluted to 1X, contains 10 mM Tris, 1 mM EDTA, pH ~8.0.

Synonym(s):

Tris-EDTA Buffer

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About This Item

UNSPSC Code:
41105319
NACRES:
NA.25

grade

Molecular Biology

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

foreign activity

DNase, RNase, protease, none detected

shipped in

ambient

storage temp.

15-25°C

General description

A 100X concentrate that, when diluted to 1X, contains 10 mM Tris, 1 mM EDTA, pH ~8.0.
Tris-ethylenediaminetetraacetic (TE) buffer is a common buffer used in molecular biology to maintain the integrity of nucleic acid during handling and storage.

Application

TE Buffer, 100X, Molecular Biology Grade – Calbiochem has been used to dilute DNA stock solutions. It has also been used to dilute thymidylate synthase (thyA) culture for colony polymerase chain reaction (PCR).

Warning

Toxicity: Irritant (B)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PRTR

Class I Designated Chemical Substances

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

574793-L:
574793-1L:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jason W Johnston et al.
Plant physiology and biochemistry : PPB, 43(9), 844-853 (2005-11-18)
HPLC analysis of nucleosides is important for determining total DNA methylation in plants and can be used to help characterise epigenetic changes during stress, growth and development. This is of particular interest for in vitro plant cultures as they are

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