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Safety Information

SML2135

Sigma-Aldrich

Biotinyl tyramide

≥97% (HPLC), powder, tyramide signal amplifier

Synonym(s):

(3aS,4S,6aR)-Hexahydro-N-[2-(4-hydroxyphenyl)ethyl]-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide, BP, Biotin-Phenol

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About This Item

Empirical Formula (Hill Notation):
C18H25N3O3S
CAS Number:
41994-02-9
Molecular Weight:
363.47
MDL number:
MFCD27953026
UNSPSC Code:
12352200
NACRES:
NA.77

product name

Biotinyl tyramide, ≥97% (HPLC)

Assay

≥97% (HPLC)

form

powder

color

white to beige

solubility

DMSO: 2 mg/mL, clear

storage temp.

−20°C

SMILES string

O=C(NCCC1=CC=C(O)C=C1)CCCC[C@@H]2SC[C@@]3([H])[C@]2([H])NC(N3)=O

Application

Biotinyl tyramide or Biotin-Phenol has been used in proximity-dependent labeling for protein identification.

Biochem/physiol Actions

Biotinyl tyramide is a reagent used for tyramide signal amplification for both immunohistochemistry (IHC) and in situ hybridization protocols and with either chromogenic or fluorescence detection. Preliminary binding of a probe is followed by secondary detection of the probe with an HRP-labeled antibody or streptavidin conjugate. Catalysis by the HRP results in the deposition of multiple biotinyl tyramide molecules in the immediate vicinity of the probe that can then be detected with a labeled streptavidin conjugate. Detection sensitivity can be 100-fold or more sensitive compared to conventional detection procedures.

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Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SML2135-50MG:
SML2135-BULK:
SML2135-VAR:
SML2135-250MG:

Certificates of Analysis (COA)

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J F Breininger et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 48(12), 1593-1599 (2000-12-02)
To increase the sensitivity of fluorescence in situ hybridization (FISH) for detection of low-abundance mRNAs, we performed FISH on cryostat sections of rat hypothalamus with biotin-labeled riboprobes to leptin receptor (ObRb) and amplified the signal by combining tyramide signal amplification
Nguyen Thi Trang et al.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 27(3), 326-331 (2015-04-10)
This study evaluated the sensitivity of biotinyl-tyramide-based in situ hybridization (TISH) method by comparison with chromogenic in situ hybridization (CISH) and immunohistochemical staining (IHC) methods. This study also determined the effect of fixative and fixation time on the detection of
Peiguo Yang et al.
Cell, 181(2), 325-345 (2020-04-18)
The mechanisms underlying ribonucleoprotein (RNP) granule assembly, including the basis for establishing and maintaining RNP granules with distinct composition, are unknown. One prominent type of RNP granule is the stress granule (SG), a dynamic and reversible cytoplasmic assembly formed in
Hagai Marmor-Kollet et al.
Molecular cell, 80(5), 876-891 (2020-11-21)
Stress granules (SGs) are cytoplasmic assemblies of proteins and non-translating mRNAs. Whereas much has been learned about SG formation, a major gap remains in understanding the compositional changes SGs undergo during normal disassembly and under disease conditions. Here, we address
Haiyang Yu et al.
Science (New York, N.Y.), 371(6529) (2020-12-19)
The RNA binding protein TDP-43 forms intranuclear or cytoplasmic aggregates in age-related neurodegenerative diseases. In this study, we found that RNA binding-deficient TDP-43 (produced by neurodegeneration-causing mutations or posttranslational acetylation in its RNA recognition motifs) drove TDP-43 demixing into intranuclear
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