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Safety Information

SHC015

Sigma-Aldrich

MISSION® pLKO.1-puro-UbC-TagFP635™ Positive Control Plasmid DNA

Contains a gene encoding TagFP635, under the control of the UbC promoter

Synonym(s):

MISSION® Control Vectors

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About This Item

MDL number:
MFCD07785395
UNSPSC Code:
12352200

product line

MISSION®

shipped in

dry ice

storage temp.

−20°C

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General description

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, we recommend producing self-inactivating replication incompetent viral particles in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The UbC-TagFP635 Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.
Silencing of the CMV promoter can be a problem in some cell types. For these cells, the ubiquitin promoter (UbC) can be a viable alternative. The MISSION UbC-TagFP635 Control Vector is a 9226 base pair lentivirus plasmid vector that contains a gene encoding TagFP635, under the control of the UbC promoter. The UbC-TagFP635 Control Vector is useful as a positive control in experiments using the MISSION shRNA library clones for applications in which an alternate promoter is necessary.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TagFP635 is a trademark of Evrogen Co.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SHC015:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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