SHM01

MISSION^® ExpressMag^® Super Magnetic Kit

Increases transduction efficiency

• UNSPSC Code: 41106609

Properties

• product line: MISSION^®
• shelf life: ≥2 yr at ~4 °C
• storage temp.: 2-8°C

Description

General description

To view protocols from Sigma collaborators for ExpressMag system visit the link below.
www.sigmaaldrich.com/mission-protocols

Application

MISSION^® ExpressMag^® Super Magnetic Kit contains all of the components needed to start enhancing your lentiviral transductions. The kit contains a 100 μL vial of ExpressMag™ Beads, enough for 63 wells of a 96 well plate. These beads bind the lentiviral particles and enable the ExpressMag™ magnetic plate to pull the virus down to the surface of cells thereby significantly increasing transduction efficiency. This product can be used in conjunction with standard sized well plates, T-25 through T-75 tissue culture flasks, and up to 5 cm dishes. The kit has been documented to enhance MISSION^® lentiviral transduction of suspension 293T as well as allowing the transduction of primary human cells such as keratinocytes at MOI as low as 1. See the technical bulletin for other cell lines transduced with the ExpressMag™ system.

Caution

Do not autoclave! Clean surface of magnet with 70% isopropyl alcohol.

Magnets should be kept away from magnetic storage devices, such as hard disks, ID cards, and credit cards, and from electronic devices and ferromagnetic materials. Persons with cardiac pacemakers should not work with these magnets.

Legal Information

ExpressMag is a registered trademark of Sigma-Aldrich Co. LLC

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

• SHM03 MISSION^® ExpressMag^® Beads, Increases transduction efficiency
• SHM04 MISSION^® ExpressMag^® Super Magnetic Plate, Increases transduction efficiency

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable