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Safety Information

SAE0005

Sigma-Aldrich

Glucose-6-phosphate Isomerase human

recombinant, expressed in E. coli

Synonym(s):

GPI, PGI

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About This Item

Enzyme Commission number:
5.3.1.9 (BRENDA, IUBMB)
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

200

Assay

≥95% (SDS-PAGE)

specific activity

≥200 units/mg protein

storage temp.

−20°C

Related Categories

Biochem/physiol Actions

Glucose-6-phosphate isomerase (GPI) is a homodimeric enzyme ubiquitously present in most organisms. GPI catalyzes the interconversion between glucose-6-phosphate and fructose-6-hosphate, the second step of the glycolytic pathway. In mammals, Glucose-6-phosphate isomerase also acts as an autocrine motility factor (AMF), a neuroleukin, and a maturation factor. GPI deficiency is the second most common erythroenzymopathy of glycolytic enzymes after pyruvate kinase deficiency. Inherited deficiency of the enzymatic activity of GPI causes hereditary nonspherocytic hemolytic anemia (HNSHA) in humans, a severe deficiency that can be associated with hydrops fetalis (a-thalassaemia), immediate neonatal death, and neurological impairment.

Physical properties

C-terminal histidine-tagged

Unit Definition

One unit will convert 1.0 mmole of D-fructose 6-phosphate to D-glucose 6-phosphate per inute at pH 7.4 at 25 °C.

Physical form

Supplied as a solution in 50 mM Tris-HCl pH 7.5, and 50% glycerol

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SAE0005-BULK:
SAE0005-20UG:
SAE0005-1000UN-PW:
SAE0005-VAR:
SAE0005-1000UN:

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Certificates of Analysis (COA)

Lot/Batch Number
11140920
065M4063V

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Soluble factors released from irradiated human mesenchymal stromal cells (MSC) may induce genetic instability in human CD34+ cells, potentially mediating hematologic disorders. Recently, we identified four key proteins in the secretome of X-ray-irradiated MSC, among them three endoplasmic reticulum proteins
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ACS nano, 8(10), 10826-10836 (2014-09-16)
The endothelium monolayer lining in the luminal side of blood vessels provides critical antithrombotic functions. Damage to these cells will expose a highly thrombogenic subendothelium, which leads to pathological vascular changes. Using combined tissue engineering and ligand-receptor targeting strategy, we
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Nature communications, 8, 15559-15559 (2017-05-31)
Inflammation and thrombosis occur together in many diseases. The leukocyte integrin Mac-1 (also known as integrin α

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