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MAK172

Sigma-Aldrich

Proteasome 20S Activity Assay Kit

sufficient for 100 fluorometric tests

Synonym(s):

20S Proteasome Detection Kit

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About This Item

EC Number:
UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

relevant disease(s)

endocrinological disorders, diabetes; neurological disorders; cancer

storage temp.

−20°C

General description

Protein degradation occurs most commonly through two major mechanisms, lysosomal degradation and proteasome mediated degradation. Lysosomal degradation occurs through proteolytic enzyme activity and is fairly non-specific. Proteasomal degradation is targeted for a specific protein through the ubiquitination of the target protein. The target protein is typically poly-ubiquitinated and activates the 26S proteasome toward proteolysis. The proteasome 26S is the most common form of the proteasome and is an ATP-dependent proteolytic complex, which contains one 20S (700-kDa) core particle structure and two 19S (700-kDa) regulatory caps.
The proteasome is responsible for the recycling of proteins and maintenance of the balance of protein synthesis and degradation. The proteasome is involved with apoptosis, DNA repair, endocytosis, and cell cycle and has been implicated in disease states such as certain types of cancer, diabetes mellitus, and Alzheimer′s disease among others making it the target for drug discovery investigations.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for measuring the chymotrypsin-like protease activity associated with the proteasome complex either in cultured cells or cell lysates.

Principle

The fluorometric proteasome 20S assay kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex in cultured cells. This kit uses LLVY-R110 as a fluorogenic indicator for proteasome activities. Cleavage of LLVY-R110 by proteasome generates strongly green fluorescent R110 that is monitored fluorimetrically at 520-530 nm with excitation at 480-500 nm. The assay is robust, and can be readily adapted for high-throughput assays to evaluate the proteasome activities or screen the inhibitors in cultured cells or in solution. The assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

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ISHL Indicated Name

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ISHL Notified Names

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Cartagena Act

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JAN Code

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Certificates of Analysis (COA)

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Javier Quero et al.
Antioxidants (Basel, Switzerland), 10(2) (2021-02-11)
The application of plant extracts for therapeutic purposes has been used in traditional medicine because plants contain bioactive compounds with beneficial properties for health. Currently, the use of these compounds that are rich in polyphenols for the treatment and prevention
The proteasome: Overview of structure and functions
Tanaka K
Proceedings of the Japan Academy. Series B, Physical and Biological Sciences, 85(1), 12-36 (2009)
Mahamud-Ur Rashid et al.
Journal of virology, 96(5), e0199021-e0199021 (2022-01-13)
Influenza A virus (IAV), an obligatory intracellular parasite, uses host cellular molecules to complete its replication cycle and suppress immune responses. Proteasome subunit alpha type 2 (PSMA2) is a cellular protein highly expressed in IAV-infected human lung epithelial A549 cells.
The Cell: A Molecular Approach (2000)
Qingtian Zhu et al.
Biochemical and biophysical research communications, 505(1), 126-133 (2018-09-22)
The proteasome is involved in the activation of NF-κB and can regulate the progression of inflammatory diseases. However, the role of proteasome in acute pancreatitis (AP) has not been demonstrated. In this study, we first observed that the protein level

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