MAK138

Autophagy Assay Kit

sufficient for 200 fluorometric tests

• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 200 fluorometric tests
• detection method: fluorometric
• relevant disease(s): cancer; immunological diseases
• storage temp.: −20°C

Description

General description

Autophagy is an evolutionarily conserved degradation process that targets long-lived proteins, organelles, and other cytoplasmic components for degradation via the lysosomal pathway. Activation of the autophagy pathway is required for multiple cellular roles, including survival during starvation, the clearance of intracellular components, development, and immunity.

Application

• Inhibitory Effect of Ursolic Acid on Proliferation and Migration of Renal Carcinoma Cells and Its Mechanism.: This study explores the inhibitory effects of ursolic acid on the proliferation and migration of renal carcinoma cells. The use of an autophagy assay kit was instrumental in demonstrating that ursolic acid induces autophagy, leading to decreased cell viability and migration. These findings highlight the potential of ursolic acid as a therapeutic agent in renal carcinoma through the modulation of autophagy (Lyu et al., 2022).

Suitability

This kit is suitable for the detection of autophagy in mammalian cells by fluorescence microscopy and fluorescence microplate readers.

Principle

The Autophagy Assay kit provides a simple and direct procedure for measuring autophagy in a variety of cell types using a proprietary fluorescent autophagosome marker (λ_ex = 333/λ_em = 518 nm).

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2