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K3753

Sigma-Aldrich

Krebs-Henseleit Buffer Modified

With 2000 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

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About This Item

UNSPSC Code:
12352207

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

General description

Krebs-Henseleit Buffer Modified is a balanced salt solution (BSS) that is used in cell culture and plays a role in providing cells with water and inorganic salts for normal cell metabolism. In addition, it provides a buffering system to maintain the optimal pH of the medium and acts as an irrigating, transporting, and diluting agent to maintain the intra- and extra-cellular osmotic balance. BSS provides glucose as an energy source for cell metabolism.

Application

Krebs-Henseleit Buffer Modified has been used:

  • to collect the rat brains for ex vivo brain slice experiments
  • to measure calcium and sodium in rat pulmonary arterial endothelial cells (PAECs)
  • to collect the uterine and forelimb blood vessel samples from horses

Quantity

Formulated to contain 9.6 grams of powder per liter of medium.

Reconstitution

Supplement with 2.1 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

K3753LF1-BIBC:
K3753-10X1L:
K3753-25KG:
K3753-50KG:
K3753-BULK:
K3753-10L:
K3753-25K:
K3753-PROC:
K3753-1L:
K3753-50K:
K3753-BIBC:
K3753-VAR:
K3753-2X5L:
K3753-50L:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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