Merck
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K4002

Sigma-Aldrich

Krebs-Ringer Bicarbonate Buffer

With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

NACRES:
NA.75

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

pH

6.1-6.7

solubility

water: 9.5 g/L, clear

storage temp.

2-8°C

General description

Bicarbonate buffered isotonic saline containing magnesium, potassium, sodium and phosphate ions.
Krebs-Ringer Bicarbonate Buffer acts as a salt solution and plays an essential role in tissue culture. A salt solution provides the cells with water and inorganic ions. It also helps in maintaining the osmotic balance and ideal pH in the medium. This bicarbonate buffered isotonic saline contains magnesium, potassium, sodium, and phosphate ions.

Application

Krebs-Ringer Bicarbonate Buffer has been used:
  • as a wash buffer for the preparation of mice primary adipocytes and lipolysis assay{
  • in the pre-perifusion for islet viability and functionality studies
  • in pituitary bioassay

Quantity

Formulated to contain 9.5 grams of powder per liter of medium.

Reconstitution

Supplement with 1.26 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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