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F5012

Sigma-Aldrich

Anti-Ferritin, Human antibody produced in rabbit

whole antiserum, liquid

Synonym(s):

Anti-FHC, Anti-FTH, Anti-FTHL6, Anti-HFE5, Anti-PIG15, Anti-PLIF

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About This Item

MDL number:
MFCD00162407
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

15 mM sodium azide

species reactivity

human

technique(s)

Ouchterlony double diffusion: 1:16

UniProt accession no.

P02794

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FTH1(2495)

General description

Ferritin, an iron-storage protein is usually present in the liver and spleen in mammals. Iron and ferritin are distributed relatively same in the eye. Ferritin is made up of heavy and light chains.

Immunogen

Ferritin isolated from normal human spleen

Application

  • Anti-Ferritin, Human antibody produced in rabbit has been used in:
  • immunohistochemistry
  • western blotting
  • immunocytochemical techniques

Anti-Ferritin, Human antibody is suitable for use in Ouchterlony double diffusion (1:16 using human spleen ferritin), and immunoelectrophoresis. The antibody may also be used in western blot (1:1000 using frozen liver homogenates) and immunoprecipitation (using 300 μg of cell lysate incubated with 6 mul of anti-ferritin for 1h at 4 ° C).
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Ferritin is an iron-storage protein that regulates iron-homeostasis in cells. While mitochondrial ferritin is a homopolymer, the cytosolic form consists of heteropolymeric L- and H-chains. Genetic mutations in ferritin L-chains have been associated with neurodegenerative disorders, whereas altered levels of ferritin have been linked to hemophagocytic lymphohistiocytosis and adult-onset Still′s disease . In immunoelectrophoresis, anti-ferritin, human antibody is specific for ferritin when used against human spleen extract and purified ferritin. No reactivity is observed against normal human serum.

Preparation Note

The antiserum has been treated to remove lipoproteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

F5012-VAR:
F5012-2ML:
F5012-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Wayne Murrell et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 233(2), 496-515 (2005-03-23)
Multipotent stem cells are thought to be responsible for the generation of new neurons in the adult brain. Neurogenesis also occurs in an accessible part of the nervous system, the olfactory mucosa. We show here that cells from human olfactory
Increased Iron Sequestration in Alveolar Macrophages in Chronic Obtructive Pulmonary Disease
Philippot Q, et al.
Testing, 9(5), e96285-e96285 (2014)
Altered expression of iron regulatory genes in cirrhotic human livers: clues to the cause of hemosiderosis
Bergmann OM, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology, 88(12), 1349-1357 (2008)
Scientific tests
Aldred EM, et al.
Pharmacology, 331-341 (2009)
Iron-Induced Retinal Damage
Handbook of nutrition, diet, and the eye, 619-626 (2014)
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