I1408

IMAC-Select Affinity Gel

(1:1 suspension in a 30% ethanol solution)

• Synonym(s): Affinity Gel
• UNSPSC Code: 41106500
• NACRES: NA.56

Properties

• form: (1:1 suspension in a 30% ethanol solution)
• shelf life: 1 yr
• technique(s): affinity chromatography: suitable
• impurities: ≤0.08 Metal Contamination (μmol/ml packed gel)
• matrix: 6% beaded agarose
• capacity: 10-30 μmol/mL binding capacity (Cu⁺²)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

Application

An immobilized metal affinity chromatography (IMAC) product that can be widely used for purifying proteins, peptides, and nucleotides of interest, most commonly proteins. IMAC-Select utilizes a non-charged linkage chemistry to connect the proprietary quadridentate chelate to the agarose bead matrix. IMAC-Select Affinity Gel is durable and can capture proteins of interest with an affinity to various metal ions such as nickel, cobalt, iron or gallium. A metal free resin allows researchers to add metal of choice, or to use the resin to remove metal contamination from a protein. IMAC-Select Affinity Gel has been used in the affinity purification of kelch-like ECH-associated protein 1 (KEAP1), Wilms tumor gene on the X chromosome (WTX) and glycoprotein G1 tail protein of the andes virus.

Physical form

1:1 suspension in a 30% ethanol solution

Preparation Note

The ethanol used to store the affiinity gel must be removed prior to use to avoid precipitation of buffer salts.The affinity gel is first washed with 1 to 2 volumes of deionized water to remove ethanol. The gel is charged with 2 to 3 volumes of a solution of the desired metal ion at a concentration of 10 mg/mL, and then equilibrated with 3 to 5 volumes of equilibration buffer.

Other Notes

Do not allow the affinity gel to remain in any buffer for extended periods of time unless it contains an anti-microbial agent such as 30% ethanol.

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P210 - P233 - P240 - P241 - P242 - P243
• Hazard Classifications: Flam. Liq. 3
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 120.2 °F - closed cup
• Flash Point(C): 49 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves