Recombinant protein purification by tag-specific affinity chromatography is a proven technology that results in highly specific recognition and purification of recombinant proteins. Various affinity purification resins are available to bind and isolate proteins containing His-tag, GST-tag, FLAG® tag, S-tag™, Strep-Tag II, and T7-tag sequences using gravity flow, low pressure, or fast protein liquid chromatography (FPLC).
A wide range of Cytiva™ Amersham chromatography media is available to purify proteins using either manual or automated methods. Prepacked formats include gravity columns, spin columns, 96-well plates, and prepacked columns for use with automated chromatography systems.
Agarose beads and affinity gels enable fusion-tagged protein purification using ligands cross-linked to beaded agarose. Good for small to large scale purification under native, denaturing, or mildly reducing conditions, these resins are suitable for gravity flow column purification, low-speed centrifugation in batch processing, and low-pressure chromatography procedures as the beads and gels are designed for lower pressures. EZview™ affinity gels incorporate a red dye that allows the user to easily differentiate pellet from supernatant, resulting in less protein loss during batch purification. Immunoprecipitation kits include affinity gels, mini-spin columns, and lysis reagent and are specially designed to allow maximal recovery of proteins from immunoprecipitates.
Superflow agarose and High-flow affinity gels use highly crosslinked agarose beads that can withstand higher pressures compared to standard agarose bead resins. This makes these resins suitable for fast protein liquid chromatography (FPLC) purification methods. Superflow agarose and high-flow affinity gels are recommended for medium to large scale purification.
Magnetic beads are used to purify recombinant fusion tagged protein using a magnetic rack or platform to separate beads from wash and elution fractions. This procedure allows for rapid processing under native or denaturing conditions within seconds, and is suitable for low throughput using single microfuge tubes or higher throughput using 96-well plates and automation.
BugBuster® purification kits combine affinity resin, wash buffers, elution buffers, and extraction reagent for convenient preparation of soluble cell extracts and affinity purification of tagged fusion proteins from E. coli.