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Safety Information

C2272

Sigma-Aldrich

Concanavalin A from Canavalia ensiformis (Jack bean)

biotin conjugate, Type IV, lyophilized powder

Synonym(s):

ConA

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

Canavalia ensiformis

Quality Level

conjugate

biotin conjugate

type

Type IV

form

lyophilized powder

composition

Protein, ~85% ε1%/280

extent of labeling

4-8 mol biotin per mol protein

impurities

Carbohydrate, essentially free

storage temp.

2-8°C

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General description

Concanavalin A is isolated from the from Canavalia ensiformis (Jack bean). It is a mitogenic lectin with a dome-like structures and is a tetrameric protein.

Application

Concanavalin A from Canavalia ensiformis (Jack bean) has been used:
  • in the colabelling of fischer rat thyroid (FRT) epithelia(104)
  • to induce autoimmune hepatitis in Cre-conditional atg7 knockout mice(105)
  • to detect glycoproteins containing α-D-mannose, α-D-glucose when used with avidin or streptavidin detection system(106)

Biochem/physiol Actions

Concanavalin A (Con A) bound to glycoenzyme has wide applications and can be dissociated in the presence of high sugar levels. Con A induces hepatic inflammation. It stimulates macrophages and promotes cytokine production. Con A also exhibits anti-hepatoma effect and could be used effectively as a vaccine for treating cancer.
Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Packaging

Package size based on protein content

Physical form

Contains sodium citrate

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

C2272-PH:
C2272-10MG:
C2272-VAR:
C2272PROC:
C2272-10MG-PW:
C2272-2MG-PW:
C2272-BULK:
C2272-BULK-N:
C2272-2MG:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hillary Claire Tunggal et al.
PloS one, 16(6), e0253265-e0253265 (2021-06-18)
A therapeutic vaccine that induces lasting control of HIV infection could eliminate the need for lifelong adherence to antiretroviral therapy. This study investigated a therapeutic DNA vaccine delivered with a single adjuvant or a novel combination of adjuvants to augment
Concanavalin A: a useful ligand for glycoenzyme immobilization?a review
Saleemuddin, M and Husain Q
Enzyme and Microbial Technology, 13(4), 290-295 (1991)
Jens Friedrichs et al.
Nature protocols, 5(7), 1353-1361 (2010-07-03)
Atomic force microscopy (AFM)-based single-cell force spectroscopy (SCFS) enables the quantitative study of cell adhesion under physiological conditions. SCFS probes adhesive interactions of single living cells with substrates such as extracellular matrix (ECM) proteins and other cells. Here we present
Glycoproteins from the cuticle of the Atlantic shore crab Carcinus maenas: I. Electrophoresis and Western-blot analysis by use of lectins
Compere P, et al.
The Biological Bulletin, 202(1), 61-73 (2002)
Atg7 knockdown augments concanavalin A-induced acute hepatitis through an ROS-mediated p38/MAPK pathway
Zhuang Y, et al.
PLoS ONE, 11(3), e0149754-e0149754 (2016)

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