S4762

Streptavidin from Streptomyces avidinii

essentially salt-free, lyophilized powder, ≥13 units/mg protein

• CAS Number: 9013-20-1
• MDL number: MFCD00082035
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• form: essentially salt-free, lyophilized powder
• Quality Level: 200
• specific activity: ≥13 units/mg protein
• mol wt: ~60 kDa
• capacity: 4 mol/mol (Biotin)
• application(s): research pathology
• storage temp.: −20°C

Description

General description

Streptavidin derives its name from its bacterial source Streptomyces avidinii and from the hen egg-white protein, avidin, which has high affinity to biotin. Its homologous core shares 33% sequence similarity with avidin, as well as sharing a common tetrameric structure. It is a crystalline tetrameric protein, with a molecular weight of 4*15000Da. It binds four molecules of biotin. Streptavidin lacks carbohydrate and sulfur-containing amino acids.

Streptavidin is a crystalline protein isolated from the bacterium Streptomyces avidinii and possesses biotin-binding ability. It has four binding sites for biotin like avidin and binds four molecules of biotin. Streptavidin is now considered as a new type of biotin-binding protein.

Application

Streptavidin from Streptomyces avidinii has been used:

• in wire functionalization
• in streptavidin capture enzyme-linked immunosorbent assay (ELISA)
• in microbubble preparation

Preparation Note

Affinity purified.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves