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189730

Sigma-Aldrich

Streptavidin

Synonym(s):

Streptavidin, Avidin, Streptomyces avidinii

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About This Item

CAS Number:
9013-20-1
MDL number:
MFCD00082035
UNSPSC Code:
41116133
NACRES:
NA.41

form

lyophilized solid

Quality Level

100

specific activity

≥10 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

shipped in

ambient

storage temp.

−20°C

General description

A high affinity biotin binding protein (Kd approximately 10-15) that is stable over wide pH and temperature ranges. Each molecule of streptavidin binds 4 molecules of biotin. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point nearer to neutrality (pI of 5.5-6.5 versus 10 for avidin). Consequently, streptavidin frequently exhibits lower levels of non-specific binding than avidin. The extinction coefficient of a 1% solution at 280 nm is 32.
A high-affinity biotin binding protein.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of streptavidin required to bind 1.0 µg of D-Biotin at pH 7.0.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Aqueous stock solutions are stable for 6 months at 4°C and for up to 2 years at -20°C.
Reconstitute in H₂O or 1 mM NaHCO₃, pH 9.0 at twice the final concentration and then dilute with an equal volume of 2X buffer.

Other Notes

Eberle, J. and Seibl, R. 1992. J. Virol. Methods 40, 347.
Gudibande, S.R., et al. 1992. Mol. Cell. Probes. 6, 495.
Nesbitt, S.A. and Horton, M.A. 1992. Anal. Biochem. 206, 267.
Roth, J., et al. 1992. Histochemistry 98, 229.
Sano, T., et al. 1992. Science 258, 120.
Scouten, W.H. and Konecny, P. 1992. Anal. Biochem. 205, 313.
Srivastava, P., et al. 1992. Cytometry 13, 711.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

189730-100MG:
189730-0MG:
189730-10MG:
189730-MG:
189730-1GM:
189730-1MG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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