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A4540

Sigma-Aldrich

Anti-Mouse IgM (μ-chain specific)−Agarose antibody produced in goat

IgG fraction of antiserum, saline suspension

Synonym(s):

Anti-Mouse IgM, Goat Anti-Mouse IgM

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

agarose conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

saline suspension

species reactivity

mouse

extent of labeling

≥5 mg per mL

technique(s)

Ouchterlony double diffusion: suitable

capacity

0.4 mg binding capacity

storage temp.

2-8°C

target post-translational modification

unmodified

General description

IgM exists as a pentamer and is secreted by peritoneal B cells. IgM is a highly conserved antibody in vertebrates and is expressed early during immune response.
IgM is a glycoprotein antibody that regulates humoral immune responses. Mouse IgM is involved in modulating B cell memory. This antibody isotype has also been implicated in the development of autoimmune responses associated with the pathogenesis of type 1 diabetes in mice.
Goat anti-mouse IgM (μ-chain specific)-agarose antibody is specific for mouse IgM when tested against purified mouse IgA, IgG (all subclasses), and IgM myeloma proteins.

Application

Anti-Mouse IgM (μ-chain specific)−Agarose antibody produced in goat has been used in agarose binding assay in primary splenic cells and in immunoprecipitation of soluble lens protein and myelogenous leukemia cell lines.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Goat anti-mouse IgM (μ-chain specific)-agarose antibody has been used for immunoprecipitation and ChIP assays. The product can also be used for Ouchterlony double diffusion applications.
To isolate chimeric proteins that were fused with the IgM heavy chain, CHO supernatant was ran over a column packed with agarose-linked goat anti-mouse IgM mu chain specific.

Biochem/physiol Actions

IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules. IgM has sites for N-linked glycosylation, with sugars namely, mannose, galactose, N-acetyl glucosamine and sialic acid IgM plays a key role in the engulfing of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM linked agarose resin have been tested for efficient conjugate binding.

Physical form

Suspension in 0.5 M NaCl containing preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

A4540-5ML:4548173185927
A4540-BULK:
A4540-25ML:4548173185903
A4540-2ML:4548173185910
A4540-VAR:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Zhang Z, et al.
The Journal of Biological Chemistry, jbc-M116 (2016)
Miltiadis Kininis et al.
Molecular and cellular biology, 29(5), 1123-1133 (2008-12-24)
Under classical models for signal-dependent transcription in eukaryotes, DNA-binding activator proteins regulate the recruitment of RNA polymerase II (Pol II) to a set of target promoters. However, recent studies, as well as our results herein, show that Pol II is
James P Hewitson et al.
Journal of immunology (Baltimore, Md. : 1950), 187(9), 4764-4777 (2011-10-04)
Heligmosomoides polygyrus is a widely used gastrointestinal helminth model of long-term chronic infection in mice, which has not been well-characterized at the antigenic level. We now identify the major targets of the murine primary Ab response as a subset of
A role for lengsin, a recruited enzyme, in terminal differentiation in the vertebrate lens
Wyatt K, et al.
The Journal of Biological Chemistry, 283(10), 6607-6615 (2008)
Juraj Adamik et al.
Cytokine, 64(1), 404-412 (2013-06-27)
Prostaglandin E2 (PGE2), IL-23 and IL-1β are implicated in inflammatory bowel disease susceptibility, likely in part by modulating IL-17 producing CD4(+) T helper (Th17) cells. To better understand how these three mediators affect Th17 cell memory responses, we characterized the

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