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A3687

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Alkaline Phosphatase Secondary Antibody, Anti Rabbit Antibody - Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat, Anti Rabbit Sigma, anti rabbit antibody

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About This Item

MDL number:
MFCD00162782
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

200

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

rabbit

technique(s)

direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody binds all rabbit Igs.

Immunogen

purified rabbit IgG as the immunogen

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Dot blot (1 paper)
Enzyme-linked immunosorbent assay (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (10 papers)
Citrullination of antithrombin by PADI4 was analyzed by ELISA using alkaline phosphatase conjugated goat anti-rabbit IgG as the secondary diluted at 1:5000 in 0.05M carbonate/bicarbonate buffer (Ph 9.6).
Goat anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody has been used for western blot and ELISA applications.the antibody can also be used for immunohistochemistry (at 1:50, using formalin-fixed, paraffin-embedded sections).
The level of hypoxyprobe binding proteins present in retinal mouse tissue was analyzed by ELISA using alkaline phosphatase conjugated goat anti-rabbit IgG as the secondary at a dilution of 1:2000 in 5% tween/PBS. Substrate used was 4-nitrophenyl phosphate disodium salt hexahydrate (Sigma).

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

A3687-VAR:
A3687PROC:
A3687-BULK:
A3687-1ML:
A3687-.5ML:
A3687-.25ML:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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G Cibelli et al.
The European journal of neuroscience, 13(7), 1339-1348 (2001-04-12)
Corticotropin-releasing factor (CRF), a neuropeptide of 41 amino acids, acts as the major physiological regulator of the basal and stress-induced release of corticotropin (ACTH), beta-endorphin and other proopiomelanocortin-derived peptides from the anterior pituitary gland. In addition to its endocrine activity
Anne-Claire Cazalé et al.
Journal of experimental botany, 60(9), 2653-2664 (2009-05-16)
Molecular chaperones of the heat shock cognate 70 kDa (HSC70) family are highly conserved in all living organisms and assist nascent protein folding in normal physiological conditions as well as in biotic and abiotic stress conditions. In the absence of
P Manders et al.
British journal of cancer, 87(7), 772-778 (2002-09-17)
The growth and metastasising capacity of solid tumours are dependent on angiogenesis. Vascular endothelial growth factor is a mediator of angiogenesis. In this study we investigated whether vascular endothelial growth factor is associated with the natural course of the disease
Éva Dóka et al.
Science advances, 2(1), e1500968-e1500968 (2016-02-05)
Hydrogen sulfide signaling involves persulfide formation at specific protein Cys residues. However, overcoming current methodological challenges in persulfide detection and elucidation of Cys regeneration mechanisms from persulfides are prerequisites for constructing a bona fide signaling model. We here establish a
Mark A Russell et al.
Islets, 5(2), 95-105 (2013-03-21)
Pro-inflammatory cytokines are important mediators of β-cell demise in type 1 diabetes, and similar mechanisms are increasingly implicated in type 2 diabetes, where a state of chronic inflammation may persist. It is likely that the actions of anti-inflammatory cytokines are
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