Skip to Content
Merck
All Photos(2)

Key Documents

View All Documentation

Safety Information

B1911

Sigma-Aldrich

BCIP®/NBT

alkaline phosphatase substrate, chromogenic, liquid

Synonym(s):

5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride solution, BCIP/NBT Alkaline Phosphatase Substrate solution

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

product name

BCIP®/NBT Liquid Substrate System, ready to use solution

Quality Level

300

form

liquid

storage temp.

2-8°C

Application

A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures.
BCIP®/NBT Liquid Substrate System has been used:
  • for staining alkaline phosphatase(ALP) in bone marrow stromal cells
  • as a substrate in western blot detection of matrix metalloproteinase-2 specific antibody
  • as a substrate for streptavidin-alkaline phosphatase in the enzyme-linked immunospot (ELISpot) assays

Caution

It is recommended to store the product between 4 - 8 °C. Do not freeze. The product may be stored at room temperature for up to one year. If a precipitate forms, the solution can be gently swirled or filtered.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

B1911-VAR:
B1911-BULK:
B1911-100ML:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Commensal microbes induce serum IgA responses that protect against polymicrobial sepsis
Wilmore JR, et al.
Cell host & microbe, 23(3), 302-311 (2018)
Nana H Overgaard et al.
Vaccine, 35(42), 5629-5636 (2017-09-10)
The relationship between the antigen dose and the quality of an immune response generated upon immunization is poorly understood. However, findings show that the immune system is indeed influenced by the antigen dose; hence underlining the importance of correctly determining
Epigenetic regulation of Runx2 transcription and osteoblast differentiation by nicotinamide phosphoribosyltransferase
Ling M, et al.
Cell & Bioscience, 7(1), 27-27 (2017)
Salma E Jiménez-Badillo et al.
Scientific reports, 7(1), 13351-13351 (2017-10-19)
The Catsper1 gene, whose expression is restricted to male germ cells, has great importance in reproductive biology because of its function in sperm motility and fertilization. We previously reported that the promoter of this gene has transcriptional activity in either
Yi Sun et al.
Veterinary research, 42, 62-62 (2011-05-17)
Babesia divergens is a tick-transmitted apicomplexan parasite for which asexual multiplication in its vertebrate hosts is restricted to erythrocytes. Current knowledge of invasion of these target cells is limited. An efficient in vitro invasion assay was set up to gain
View All Related Papers

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service