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AP132A

Sigma-Aldrich

Goat Anti-Rabbit IgG Antibody, Alkaline Phosphatase conjugate

0.6 mg/mL, Chemicon®

Synonym(s):

Alkaline Phosphatase Anti-Rabbit, Anti-Rabbit IgG AP

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

goat

Quality Level

100

conjugate

alkaline phosphatase conjugate

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

rabbit

manufacturer/tradename

Chemicon®

concentration

0.6 mg/mL

technique(s)

ELISA: suitable
western blot: suitable

shipped in

wet ice

target post-translational modification

unmodified

General description

Goat anti Rabbit IgG affinity purified antibody, Alkaline Phosphatase conjugated.
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Application

ELISA: 1:5,000-1:50,000

Western Blot: 1:5,000-1:50,000

Optimal working dilutions must be determined by the end user.
Goat anti-Rabbit IgG Antibody, Alkaline Phosphatase conjugate is an antibody against Rabbit IgG for use in ELISA & WB.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Linkage

Replaces: AP158A

Physical form

ImmunoAffinity Purified
RECONSTITUTION:

Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge or vortex this product if it is not completely clear after standing for 1-2 hours at room temperature.

Storage and Stability

Store lyophilized material at 2-8°C until opened. After reconstitution this product is stable for several weeks at 2-8°C as an undiluted liquid. DO NOT FREEZE. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol and store at 2-8°C for up to 12 months. Please note that the concentration of protein and buffer salts will decrease to one-half of the original after adding glycerol.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictograms

Skull and crossbones
GHS06

Signal Word

Danger

Hazard Statements

H302 + H332,H311,H412

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Poisonous substance

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

AP132A:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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