Merck
All Photos(1)

08069

Millipore

Modified tryptone soy broth

suitable for microbiology, NutriSelect® Plus

Synonym(s):
Modified soybean bile broth base, Tryptic soy broth, modified, mTSB
NACRES:
NA.85

Agency

according to ISO 16654:2001

Quality Level

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

bile salts mixture, 1.5 g/L
casein enzymic hydrolysate, 17 g/L
dipotassium hydrogen phosphate, 4 g/L
D-glucose, 2.5 g/L
papaic digest of soyabean meal, 3 g/L
sodium chloride, 5 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.3±0.2 (25 °C)

application(s)

food and beverages
pathogen testing
vitamins, nutraceuticals, and natural products

microbiology

suitability

selective for enterovirulent E. coli (EEC)

Application

Modified tryptone soy broth is recommended as an enrichment medium for the detection of Escherichia coli O157:H7 from food and animal feeding stuffs.

Preparation Note

Suspend 33 grams of Modified tryptone soy broth in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 minutes. Cool to 45-50 °C and aseptically add rehydrated contents of 1 vial of VCC Selective Supplement (Cat. No. No. 80704). Mix well and dispense as desired.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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