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Safety Information

T8907

Millipore

Tryptic Soy Broth - Dehydrated Culture Media

NutriSelect® Basic, powder, suitable for microbiology

Synonym(s):

Soybean Casein digest Broth, Casein Soya Broth, CASO Broth, TSB, CASO Broth, Casein Soya Broth, Soybean Casein digest Broth, TSB, Tryptone Soya Broth

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About This Item

MDL number:
MFCD00132536
UNSPSC Code:
41171605
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

quality

Microbiologically tested.

packaging

poly bottle of 1 kg
poly bottle of 500 g

manufacturer/tradename

NutriSelect® Basic

application(s)

environmental
food and beverages
pathogen testing
pharmaceutical
microbiology

suitability

bacteria

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General description

Tryptic Soy Broth is a general-purpose liquid medium used in qualitative procedures for the sterility testing, enrichment, and cultivation of fastidious microorganisms. The formulation contains a combination of casein and soy peptones supplying organic nitrogen, particularly amino acids and long-chained peptides. Sodium chloride maintains the osmotic equilibrium and dextrose serves as a source of carbohydrate and energy.

Application

Tryptic Soy Broth is a widely used medium for culturing aerobic gram-positive, gram-negative bacteria, fungus, routine blood cultures, and microbial biofilms. It is also been used as a growth medium to culture Staphylococcus aureus strains and as a growth medium for E.coli in the bacterial-killing assay (BKA) with bird plasma samples.

Components

Casein (pancreatic digest) 17 g/L
Soya peptone (papaic digest) 3 g/L
Sodium chloride 5 g/L
Dipotassium phosphate 2.5 g/L
Dextrose 2.5 g/L

Preparation Note

Suspend 30 g of dehydrated media in 1 litre of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Cool to 45-50°C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

T8907-1KG:
T8907-10KG:
T8907-500G:
T8907-BULK:
T8907-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCL5206
SLCL3420
SLCK9081
SLCK0913
SLCD9263

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Sisi Gao et al.
The Journal of experimental biology, 220(Pt 2), 322-327 (2016-11-05)
Stress-induced effects on innate immune activity in wild birds have been difficult to predict. These difficulties may arise from the frequent assumptions that (1) the stress response influences different components of the immune response similarly, (2) stress-induced effects do not
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Archives of microbiology, 203(1), 269-277 (2020-09-13)
Bacterial biofilms are related to various dental and periodontal infectious diseases, and the characterization of this biological structure with micro-computed tomography (micro-CT) may offer valuable information for clinical and research applications. In this study, we aimed to develop a model
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For most tissue engineering applications, surface modification and sterilization of polymers are critical aspects determining the implant success. The first part of this study is thus dedicated to modifying polycaprolactone (PCL) surfaces via plasma treatment using a medium pressure dielectric
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