Merck
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76704

Millipore

mTSB Broth with Novobiocin

suitable for microbiology, NutriSelect® Plus, selective for enterovirulent E. coli (EEC)

Synonym(s):
Modified Tryptic Soy Broth with Novobiocin, Modified Tryptic Soy Broth; EHEC enrichment broth
NACRES:
NA.85

Quality Level

Agency

according to ISO 16654:2001

sterility

non-sterile

form

powder

shelf life

limited, expiry date on the label

composition

bile salts No. 3, 1.5 g/L
di-potassium hydrogen phosphate, 4.0 g/L
D(+)-glucose, 2.5 g/L
novobiocin, 0.02 g/L
peptone from casein, 17.0 g/L
peptone from soymeal, 3.0 g/L
sodium chloride, 5.0 g/L

manufacturer/tradename

NutriSelect® Plus

packaging

pkg of -500 g

storage condition

(Dry place, tightly sealed containers)

technique(s)

microbiological culture: suitable

color

faint brown

final pH

7.3±0.2 (25 °C)

application(s)

agriculture
food and beverages
pathogen testing

microbiology

storage temp.

2-25°C

suitability

selective for Escherichia coli
selective for coliforms
selective for enterovirulent E. coli (EEC)

General description

mTSB Broth with Novobiocin is suitable for the selective enrichment of enterohemorrhagic Escherichia coli (EHEC) in foods intended for human and animal consumption. The peptone provides amino acids and other complex nitrogenous substances. Glucose is a fermentable carbohydrate. Sodium chloride delivers osmotic balance. Bile salts inhibit the growth of Gram-positive accompanying bacterial flora. Di-potassium hydrogen phosphate is the buffering agent to stabilize the pH.mTSB is made selective for E. coli O157:H7 by adding bile salts in the media along with Novobiocin supplementation.

Application

mTSB Broth with Novobiocin is recommended for the selective enrichment of enterohemorrhagic E. coli (EHEC) in foods, especially meat and poultry products.The medium meets the requirements of the DIN Norm 10167 for the detection of E. coli serotype O157:H7 in foods. It is also recommended by the FDA-BAM as a method for the isolation of enterohemorrhagic E. coli (EHEC).

Preparation Note

Suspend 33 g in 1 litre of distilled water, autoclave at 121°C for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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