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Safety Information

SCT263

Sigma-Aldrich

BioTracker™ Mitophagy Detection Kit

Human

Synonym(s):

mitochondrial degradation probe, mitophagy detection, mitophagy live cell kit, mitophagy probe

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About This Item

UNSPSC Code:
41106514
NACRES:
NB.12

product name

BioTracker™ Mitophagy Detection Kit,

biological source

human

Quality Level

100

form

lyophilized solid

packaging

vial of 1 kit

manufacturer/tradename

Millipore

technique(s)

cell culture | stem cell: suitable

shipped in

2-8°C

storage temp.

2-8°C

General description

Two-probe kit for detection of mitophagy and confirmation of fusion with the lysosome useful for study of PD, AD, and other diseases where mitochondrial dysfunction and processing is known to be critical.

SPECTRAL PROPERTIES
Mitophagy probe:
λex: 530 nm
λem: 700 nm
Lysosome probe:
λex: 398 nm
λem: 525 nm


A 100 uM stock solution of the mitophagy probe is created by dissolving 50 uL DMSO to one tube (5 ug) of mitophagy dye. A typical working solution concentration is 1 nM.
A 1 mM stock solution of the ysosome dye is created by resuspending the contents of the lysosome dye in 55 uL DMSO. A typical concentration for the working solution is 1 uM.

Features and Benefits

The SCT263 kit is composed of a mitophagy detection probe and a lysosome probe. The mitophagy dye accumulates in intact mitochondria where it is immobilized by chemical bonding and emits weak fluorescence. Upon mitophagy and fusion with the lysosome, damaged mitochondria increase in fluorescence in tandem with decreased pH.

Target description

Mitochondria are cytoplasmic organelles that play a crucial cellular role in the production of energy for cell viability and function. Recently, mitophagy has been shown to be related to Alzheimer′s and to Parkinson′s diseases and induced by the accumulation of depolarized mitochondria. Mitophagy serves as a specific elimination system in which dysfunctional mitochondria damaged by oxidative stress in DNA are sequestered into the autophagosome, fused to the lysosome, and degraded by digestion.

Physical form

purple solid (mitophagy probe), yellow solid (lysosome probe)

Storage and Stability

Store at 2 - 8 °C, protected from light.

Other Notes

This live cell probe mitophagy detection kit includes a probe for mitochondrial degradation (far red) plus a probe to confirm association of degraded mitochondria with the lysosome (green). The kit can be used in conjunction with SCT272, BioTracker Red Mitochondria Live Cell Probe.

Legal Information

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SCT263:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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