R6148

RNase A solution

• Synonym(s): GenElute^™ RNAse A
• UNSPSC Code: 12352204
• NACRES: NA.52

Properties

• biological source: bovine pancreas
• Quality Level: 200
• form: liquid
• specific activity: 1500 units/mL; 3000 units/mL
• concentration: 20 mg/mL in Tris buffer
• technique(s): DNA purification: suitable
• storage temp.: room temp
• Gene Information: bovine ... RNASE1(282340) , RNASE1(280930)

Description

General description

Ribonuclease A (RNase A), a protein composed of three α helices and seven β strands arranged in two "lobes” holds significance as the prototypical domain-swapped protein. It is known for its diverse oligomeric states. The RNase A family derives its name from bovine pancreatic RNase A and is specific to vertebrates. In humans, this family encompasses eight functional members, showcasing its biological relevance.
For sample preparation in GenElute^™ kits.

Application

RNase A solution has been used:

• in apoptosis assay of Hep-2 cells
• for the colorimetric quantification of methylated DNA
• for DNA and RNA extraction
• in DNA laddering analysis
• in cell cycle analysis
• as a component in phosphate-buffered saline (PBS) to incubate cells for flow cytometry

Biochem/physiol Actions

Ribonuclease A or bovine pancreatic ribonuclease A (RNase A) enzyme is an endoribonuclease that displays a specific ability to cleave single-stranded RNA on the 3′ side of pyrimidine residues. This endonuclease with 124 amino acids plays a key role in gene duplication, cellular metabolism, and cancer therapy. Its overexpression is closely associated with several types of cancer. Hence, RNase A has the potential to be a promising new biomarker for the diagnosis of malignant tumors and is also used as a specific target for new drug discovery. RNase A is commonly used for the isolation of plasmid DNA.

Preparation Note

For usage requirements, please refer to the kit procedure.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable